Van Mil, ACCM and Greyling, A and Zock, PL and Geleijnse, JM and Hopman, MT and Mensink, RP and Reesink, KD and Green, DJ and Ghiadoni, L and Thijssen, DHJ (2016) Impact of volunteer-related and methodology-related factors on the reproducibility of brachial artery flow-mediated vasodilation: Analysis of 672 individual repeated measurements. Journal of Hypertension, 34 (9). pp. 1738-1745. ISSN 0263-6352
van Mil fmd reproducibility_JHypertension - clean version.pdf - Accepted Version
Restricted to Repository staff only until 1 September 2017.
Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.Objectives: Brachial artery flow-mediated dilation (FMD) is a popular technique to examine endothelial function in humans. Identifying volunteer and methodological factors related to variation in FMD is important to improve measurement accuracy and applicability. Methods: Volunteer-related and methodology-related parameters were collected in 672 volunteers from eight affiliated centres worldwide who underwent repeated measures of FMD. All centres adopted contemporary expert-consensus guidelines for FMD assessment. After calculating the coefficient of variation (%) of the FMD for each individual, we constructed quartiles (n = 168 per quartile). Based on two regression models (volunteer-related factors and methodology-related factors), statistically significant components of these two models were added to a final regression model (calculated as β-coefficient and R 2). This allowed us to identify factors that independently contributed to the variation in FMD%. Results: Median coefficient of variation was 17.5%, with healthy volunteers demonstrating a coefficient of variation 9.3%. Regression models revealed age (β = 0.248, P < 0.001), hypertension (β = 0.104, P < 0.001), dyslipidemia (β = 0.331, P < 0.001), time between measurements (β = 0.318, P < 0.001), lab experience (β = -0.133, P < 0.001) and baseline FMD% (β = 0.082, P < 0.05) as contributors to the coefficient of variation. After including all significant factors in the final model, we found that time between measurements, hypertension, baseline FMD% and lab experience with FMD independently predicted brachial artery variability (total R2 = 0.202). Conclusion: Although FMD% showed good reproducibility, larger variation was observed in conditions with longer time between measurements, hypertension, less experience and lower baseline FMD%. Accounting for these factors may improve FMD% variability.
|Additional Information:||This is not the published version|
|Uncontrolled Keywords:||1103 Clinical Sciences, 1102 Cardiovascular Medicine And Haematology|
|Subjects:||R Medicine > R Medicine (General)|
|Divisions:||Sport & Exercise Sciences|
|Publisher:||Lippincott, Williams & Wilkins|
|Date Deposited:||22 Aug 2016 11:08|
|Last Modified:||22 Aug 2016 11:08|
|DOI or Identification number:||10.1097/HJH.0000000000001012|
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