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Impact of volunteer-related and methodology-related factors on the reproducibility of brachial artery flow-mediated vasodilation: Analysis of 672 individual repeated measurements

Van Mil, ACCM and Greyling, A and Zock, PL and Geleijnse, JM and Hopman, MT and Mensink, RP and Reesink, KD and Green, DJ and Ghiadoni, L and Thijssen, DHJ (2016) Impact of volunteer-related and methodology-related factors on the reproducibility of brachial artery flow-mediated vasodilation: Analysis of 672 individual repeated measurements. Journal of Hypertension, 34 (9). pp. 1738-1745. ISSN 0263-6352

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Abstract

Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.Objectives: Brachial artery flow-mediated dilation (FMD) is a popular technique to examine endothelial function in humans. Identifying volunteer and methodological factors related to variation in FMD is important to improve measurement accuracy and applicability. Methods: Volunteer-related and methodology-related parameters were collected in 672 volunteers from eight affiliated centres worldwide who underwent repeated measures of FMD. All centres adopted contemporary expert-consensus guidelines for FMD assessment. After calculating the coefficient of variation (%) of the FMD for each individual, we constructed quartiles (n = 168 per quartile). Based on two regression models (volunteer-related factors and methodology-related factors), statistically significant components of these two models were added to a final regression model (calculated as β-coefficient and R 2). This allowed us to identify factors that independently contributed to the variation in FMD%. Results: Median coefficient of variation was 17.5%, with healthy volunteers demonstrating a coefficient of variation 9.3%. Regression models revealed age (β = 0.248, P < 0.001), hypertension (β = 0.104, P < 0.001), dyslipidemia (β = 0.331, P < 0.001), time between measurements (β = 0.318, P < 0.001), lab experience (β = -0.133, P < 0.001) and baseline FMD% (β = 0.082, P < 0.05) as contributors to the coefficient of variation. After including all significant factors in the final model, we found that time between measurements, hypertension, baseline FMD% and lab experience with FMD independently predicted brachial artery variability (total R2 = 0.202). Conclusion: Although FMD% showed good reproducibility, larger variation was observed in conditions with longer time between measurements, hypertension, less experience and lower baseline FMD%. Accounting for these factors may improve FMD% variability.

Item Type: Article
Additional Information: This is not the published version
Uncontrolled Keywords: 1103 Clinical Sciences, 1102 Cardiovascular Medicine And Haematology
Subjects: R Medicine > R Medicine (General)
Divisions: Sport & Exercise Sciences
Publisher: Lippincott, Williams & Wilkins
Date Deposited: 22 Aug 2016 11:08
Last Modified: 12 Sep 2017 21:45
DOI or Identification number: 10.1097/HJH.0000000000001012
URI: http://researchonline.ljmu.ac.uk/id/eprint/4040

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