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A demonstration of athermal effects of continuous microwave irradiation on the growth and antibiotic sensitivity of Pseudomonas aeruginosa PAO1.

Nakouti, I and Hobbs, G and Teethaisong, Y and Phipps, D (2017) A demonstration of athermal effects of continuous microwave irradiation on the growth and antibiotic sensitivity of Pseudomonas aeruginosa PAO1. Biotechnology Progress, 33 (1). pp. 37-44. ISSN 8756-7938

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s1-ln24921873-26103616-1939656818Hwf-1664916733IdV-158525184924921873PDF_HI0001.pdf - Accepted Version
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Abstract

Stress, caused by exposure to microwaves (2.45GHz) at constant temperature (37± 0.5(o) C), alters the growth profile of Pseudomonas aeruginosa PAO1. In the absence of microwave treatment a simple, highly reproducible growth curve was observed over 24 hours or more. Microwave treatment caused no reduction in growth during the first 6 hours, but at a later stage (>12hours) the growth was markedly different to the controls. Secondary growth, typical of the presence of persisters clearly became apparent, as judged by both the dissolved oxygen and the cell density profiles. These treated cells showed distinct morphological changes, but on re-growth these cells reverted to normal. The Microwave Induced Persisters were subject to antibiotic challenge (tobramycin) and showed increased sensitivity when compared to the un-stressed planktonic cells. This is in marked contrast to antibiotic induced persisters which show increased resistance. This provides evidence for both a non-thermal effect of microwaves and a previously undescribed route to a novel form of antibiotic susceptible persister cells. This article is protected by copyright. All rights reserved.

Item Type: Article
Additional Information: This is the accepted version of the following article: Nakouti, I., Hobbs, G., Teethaisong, Y. and Phipps, D. (2017), A demonstration of athermal effects of continuous microwave irradiation on the growth and antibiotic sensitivity of Pseudomonas aeruginosa PAO1. Biotechnol Progress, 33: 37–44. doi:10.1002/btpr.2392, which has been published in final form at http://dx.doi.org/10.1002/btpr.2392
Uncontrolled Keywords: 06 Biological Sciences, 09 Engineering, 10 Technology
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Built Environment
Pharmacy & Biomolecular Sciences
Publisher: Wiley
Related URLs:
Date Deposited: 09 Nov 2016 11:01
Last Modified: 21 Apr 2017 11:08
DOI or Identification number: 10.1002/btpr.2392
URI: http://researchonline.ljmu.ac.uk/id/eprint/4761

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