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Oxidative stress and immunosenescence in spleen of obese mice can be reversed by 2-hydroxyoleic acid.

Gheorghe, A and Pérez de Heredia, F and Hunsche, C and Redondo, N and Díaz, LE and Hernández, O and Marcos, A and De la Fuente, M (2017) Oxidative stress and immunosenescence in spleen of obese mice can be reversed by 2-hydroxyoleic acid. Experimental Physiology. ISSN 1469-445X

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Abstract

We aimed to investigate the effects of obesity on oxidative stress and leukocyte function in spleen of mice, and to assess whether supplementation with 2-hydroxyoleic acid (2-OHOA) or n-3 polyunsaturated fatty acids (PUFA) could reverse those effects. Female ICR/CD1 mice (8 weeks old, n = 24) received an obesogenic diet (22% fat for 4 weeks and 60% fat for 14 weeks). After 6 weeks, mice were split in three groups (n = 8/group): no supplementation, 2-OHOA supplementation (1500 mg kg(-1) ) and n-3 PUFA supplementation (EPA + DHA, 3000 mg kg(-1) diet). Eight mice were fed standard diet for the whole duration of the study (control group). At the end of the experiment, the following variables were assessed in spleens: levels of reduced (GSH) and oxidized (GSSG) glutathione, GSH/GSSG, xanthine oxidase (XO) activity, lipid peroxidation, lymphocyte chemotaxis, natural killer (NK) activity and mitogen (ConA and LPS)-induced lymphocyte proliferation. Obese animals presented higher GSSG levels (P = 0.003), GSSG/GSH ratio (P = 0.013), lipid peroxidation (P = 0.004), XO activity (P = 0.015) and lymphocyte chemotaxis (P < 0.001), and lower NK activity (P = 0.003) and proliferation in response to ConA (P < 0.001) than controls. 2-OHOA reversed totally or partially most of the changes (body weight, fat content, GSSG levels, GSH/GSSG, lipid peroxidation, chemotaxis and proliferation, all P < 0.05), while n-3 PUFA reversed the increase in XO activity (P = 0.032). In conclusion, 2-OHOA, and to a lesser extent n-3 PUFA, could ameliorate the oxidative stress and alteration of leukocyte function in spleen of obese mice. Our findings support a link between obesity and immunosenescence and suggest a potential therapeutic tool for obesity-related immune dysfunction. This article is protected by copyright. All rights reserved.

Item Type: Article
Additional Information: This is the accepted version of the following article: Gheorghe, A., Pérez de Heredia, F., Hunsche, C., Redondo, N., Díaz, L. E., Hernández, O., Marcos, A. and De la Fuente, M. (2017), Oxidative stress and immunosenescence in spleen of obese mice can be reversed by 2-hydroxyoleic acid. Exp Physiol. Accepted Author Manuscript. doi:10.1113/EP086157, which has been published in final form at http://dx.doi.org/10.1113/EP086157
Uncontrolled Keywords: 0606 Physiology, 1116 Medical Physiology, 1106 Human Movement And Sports Science
Subjects: Q Science > QD Chemistry
R Medicine > RM Therapeutics. Pharmacology
Divisions: Natural Sciences and Psychology
Publisher: Wiley
Related URLs:
Date Deposited: 27 Apr 2017 08:32
Last Modified: 27 Apr 2017 08:32
DOI or Identification number: 10.1113/EP086157
URI: http://researchonline.ljmu.ac.uk/id/eprint/6303

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