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Label-free profiling of white adipose tissue of rats exhibiting high or low levels of intrinsic exercise capacity

Bowden-Davies, K and Connolly, J and Burghardt, P and Koch, LG and Britton, SL and Burniston, JG (2015) Label-free profiling of white adipose tissue of rats exhibiting high or low levels of intrinsic exercise capacity. Protemics, 15 (13). pp. 2342-2349. ISSN 1615-9853

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Abstract

Divergent selection has created rat phenotypes of high- and low-capacity runners (HCR and LCR, respectively) that have differences in aerobic capacity and correlated traits such as adiposity. We analyzed visceral adipose tissue of HCR and LCR using label-free high-definition MS (elevated energy) profiling. The running capacity of HCR was ninefold greater than LCR. Proteome profiling encompassed 448 proteins and detected 30 significant (p <0.05; false discovery rate <10%, calculated using q-values) differences. Approximately half of the proteins analyzed were of mitochondrial origin, but there were no significant differences in the abundance of proteins involved in aerobic metabolism. Instead, adipose tissue of LCR rats exhibited greater abundances of proteins associated with adipogenesis (e.g. cathepsin D), ER stress (e.g. 78 kDa glucose response protein), and inflammation (e.g. Ig gamma-2B chain C region). Whereas the abundance antioxidant enzymes such as superoxide dismutase [Cu-Zn] was greater in HCR tissue. Putative adipokines were also detected, in particular protein S100-B, was 431% more abundant in LCR adipose tissue. These findings reveal low running capacity is associated with a pathological profile in visceral adipose tissue proteome despite no detectable differences in mitochondrial protein abundance.

Item Type: Article
Additional Information: This is the accepted version of the following article: Bowden-Davies, K., Connolly, J., Burghardt, P., Koch, L. G., Britton, S. L. and Burniston, J. G. (2015), Label-free profiling of white adipose tissue of rats exhibiting high or low levels of intrinsic exercise capacity. Proteomics, 15: 2342–2349. doi:10.1002/pmic.201400537 which has been published in final form at http://dx.doi.org/10.1002/pmic.201400537
Uncontrolled Keywords: 06 Biological Sciences, 08 Information And Computing Sciences, 11 Medical And Health Sciences
Subjects: R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Sport & Exercise Sciences
Publisher: Wiley
Related URLs:
Date Deposited: 10 Oct 2017 09:15
Last Modified: 10 Oct 2017 09:15
DOI or Identification number: 10.1002/pmic.201400537
URI: http://researchonline.ljmu.ac.uk/id/eprint/7310

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