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Application of Box-Behnken Design for Ultrasound- Assisted Extraction and Recycling Preparative HPLC for Isolation of Anthraquinones from Cassia singueana

Jibril, S, Basar, N, Sirat, HM, Wahab, RA, Mahat, NA, Nahar, L and Sarker, SD Application of Box-Behnken Design for Ultrasound- Assisted Extraction and Recycling Preparative HPLC for Isolation of Anthraquinones from Cassia singueana. Phytochemical Analysis. ISSN 0958-0344 (Accepted)

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Abstract

Introduction – Cassia singueana Del. (Fabaceae) is a rare medicinal plant used in the traditional medicine preparations to treat various ailments. The root of C. singueana is a rich source of anthraquinones that possess anticancer, antibacterial and antifungal properties.
Objective – The objective of this study was to develop ultrasound-assisted extraction (UAE) method for achieving a high extraction yield of anthraquinones using the response surface methodology (RSM), Box-Behnken Design (BBD), and a recycling preparative HPLC protocol for isolation of anthraquinones from C. singueana.
Methodology – Optimisation of UAE was performed using the Box-Behnken experimental design. Recycling preparative HPLC was employed to isolate anthraquinones from the root extract of C. singueana.
Results - The BBD was well-described by a quadratic polynomial model (R2 = 0.9751). The predicted optimal UAE conditions for a high extraction yield were obtained at: extraction time 25.00 min, temperature 50C and solvent-sample ratio of 10 mL/g. Under the predicted conditions, the experimental value (1.65 ± 0.07%) closely agreed to the predicted yield (1.64%). The obtained crude extract of C. singueana root was subsequently purified to afford eight anthraquinones.
Conclusion - The extraction protocol described here is suitable for large-scale extraction of anthraquinones from plant extracts.

Item Type: Article
Uncontrolled Keywords: 03 Chemical Sciences, 11 Medical And Health Sciences, 06 Biological Sciences
Subjects: R Medicine > RM Therapeutics. Pharmacology
R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Wiley
Date Deposited: 14 Aug 2018 11:05
Last Modified: 02 Oct 2018 01:40
URI: http://researchonline.ljmu.ac.uk/id/eprint/9089

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