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Phytochemical profiling and evaluation of modified resazurin microtiter plate assay of the roots of Trillium govanianum

Khan, KM, Sarker, SD, Khan, GA, Saleem, H, Khan, SA and Mannan, A (2019) Phytochemical profiling and evaluation of modified resazurin microtiter plate assay of the roots of Trillium govanianum. Natural Product Research. ISSN 1478-6419

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Abstract

Trillium govanianum Wall. ex D. Don (Melanthiaceae alt. Trilliaceae), is native to the Himalayas. The present study, for the first time, was undertaken to explore the antimicrobial potential, to determine the minimum inhibitory concentration (MIC) values of the methanol extract of the roots of Trillium govanianum and its solid phase extraction (SPE) fractions by using resazurin microtiter assay (REMA) against Gram positive and Gram negative bacterial registered strains and to carry out phytochemical analysis. The remarkable amount of gallic acid equivalent phenolic and quercetin equivalent flavonoid content was manifested by MeOH extract (20.27±3.03 mg GAE/ g DW and 9.25± 0.50 mg QE/ g DW respectively). The GC/MS analysis revealed the presence saturated and unsaturated components. Considerable level of antibacterial potential against Gram-positive bacteria (MIC: 2.5-0.009 mg/mL) than against Gram-negative bacteria (MIC: 2.5-0.165 mg/mL). The use of microtiter plates has the advantage of lower cost, fast and quantitative results.

Item Type: Article
Additional Information: This is an Accepted Manuscript of an article published by Taylor & Francis in Natural Product Research on 24/03/2019, available online: http://www.tandfonline.com/10.1080/14786419.2019.1590716
Uncontrolled Keywords: MD Multidisciplinary
Subjects: R Medicine > RM Therapeutics. Pharmacology
R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Taylor & Francis
Date Deposited: 01 Mar 2019 09:24
Last Modified: 24 Mar 2020 00:50
DOI or Identification number: 10.1080/14786419.2019.1590716
URI: https://researchonline.ljmu.ac.uk/id/eprint/10230

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