Facial reconstruction

Search LJMU Research Online

Browse Repository | Browse E-Theses

In Vitro Evaluation of Eudragit Matrices for Oral Delivery of BCG Vaccine to Animals

Saleem, IY, Coombes, AGA and Chambers, MA (2019) In Vitro Evaluation of Eudragit Matrices for Oral Delivery of BCG Vaccine to Animals. Pharmaceutics, 11 (6). ISSN 1999-4923

[img]
Preview
Text
pharmaceutics-11-00270.pdf - Published Version
Available under License Creative Commons Attribution.

Download (2MB) | Preview
Open Access URL: https://www.mdpi.com/1999-4923/11/6/270 (Published version)

Abstract

Bacillus Calmette–Guérin (BCG) vaccine is the only licensed vaccine against tuberculosis (TB) in humans and animals. It is most commonly administered parenterally, but oral delivery is highly advantageous for the immunisation of cattle and wildlife hosts of TB in particular. Since BCG is susceptible to inactivation in the gut, vaccine formulations were prepared from suspensions of Eudragit L100 copolymer powder and BCG in phosphate-buffered saline (PBS), containing Tween® 80, with and without the addition of mannitol or trehalose. Samples were frozen at −20 °C, freeze-dried and the lyophilised powders were compressed to produce BCG–Eudragit matrices. Production of the dried powders resulted in a reduction in BCG viability. Substantial losses in viability occurred at the initial formulation stage and at the stage of powder compaction. Data indicated that the Eudragit matrix protected BCG against simulated gastric fluid (SGF). The matrices remained intact in SGF and dissolved completely in simulated intestinal fluid (SIF) within three hours. The inclusion of mannitol or trehalose in the matrix provided additional protection to BCG during freeze-drying. Control needs to be exercised over BCG aggregation, freeze-drying and powder compaction conditions to minimise physical damage of the bacterial cell wall and maximise the viability of oral BCG vaccines prepared by dry powder compaction.

Item Type: Article
Subjects: Q Science > QL Zoology
R Medicine > RM Therapeutics. Pharmacology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: MDPI
Date Deposited: 14 Jun 2019 11:54
Last Modified: 14 Jun 2019 12:00
DOI or Identification number: 10.3390/pharmaceutics11060270
URI: http://researchonline.ljmu.ac.uk/id/eprint/10895

Actions (login required)

View Item View Item