Hughes, ATL, Guilding, C, Lennox, L, Samuels, RE, McMahon, DG and Piggins, HD (2008) Live imaging of altered period1 expression in the suprachiasmatic nuclei of Vipr2-/- mice. Journal of Neurochemistry, 106 (4). pp. 1646-1657. ISSN 0022-3042

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Abstract

Vasoactive intestinal polypeptide and its receptor, VPAC2, play important roles in the functioning of the brain’s circadian clock in the suprachiasmatic nuclei (SCN). Mice lacking VPAC2 receptors (Vipr2−/−) show altered circadian rhythms in locomotor behavior, neuronal firing rate, and clock gene expression, however, the nature of molecular oscillations in individual cells is unclear. Here, we used real‐time confocal imaging of a destabilized green fluorescent protein (GFP) reporter to track the expression of the core clock gene Per1 in live SCN‐containing brain slices from wild‐type (WT) and Vipr2−/− mice. Rhythms in Per1‐driven GFP were detected in WT and Vipr2−/− cells, though a significantly lower number and proportion of cells in Vipr2−/− slices expressed detectable rhythms. Further, Vipr2−/− cells expressed significantly lower amplitude oscillations than WT cells. Within each slice, the phases of WT cells were synchronized whereas cells in Vipr2−/− slices were poorly synchronized. Most GFP‐expressing cells, from both genotypes, expressed neither vasopressin nor vasoactive intestinal polypeptide. Pharmacological blockade of VPAC2 receptors in WT SCN slices partially mimicked the Vipr2−/− phenotype. These data demonstrate that intercellular communication via the VPAC2 receptor is important for SCN neurons to sustain robust, synchronous oscillations in clock gene expression.

Item Type:	Article
Uncontrolled Keywords:	1109 Neurosciences
Subjects:	B Philosophy. Psychology. Religion > BF Psychology R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Divisions:	Natural Sciences & Psychology (closed 31 Aug 19)
Publisher:	Wiley
Related URLs:	Author
Date Deposited:	05 Sep 2019 10:14
Last Modified:	03 Sep 2021 22:54
DOI or ID number:	10.1111/j.1471-4159.2008.05520.x
URI:	https://researchonline.ljmu.ac.uk/id/eprint/11279

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