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Casey, DR, Wylie, D, Gallo, J, Dent, MR, Salehi-Reyhani, A, Wilson, R, Brooks, NJ, Long, NJ, Willison, KR, Klug, DR, Neil, MAA, Neale, SL, Cooper, JM and Ces, O (2015) A Novel, All-Optical Tool for Controllable and Non- Destructive Poration of Cells with Single-Micron Resolution. In: Optics in the Life Sciences . (Optics in the Life Sciences, 12th- 15th April 2015, Vancouver, BC).
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Photothermal poration - D Casey - 3-page summary for OSA Vancouver - updated.pdf - Accepted Version Download (192kB) | Preview |
Abstract
We demonstrate controllable poration within ≈1 µm regions of individual cells, mediated by a near-IR laser interacting with thin-layer amorphous silicon substrates. This technique will allow new experiments in single-cell biology, particularly in neuroscience. As our understanding of the fundamental mechanistic processes underpinning biology expands, so does the need for high-precision tools to allow the dissection of the heterogeneity and stochastic processes that dominate at the single- and sub-cellular level. Here, we demonstrate a highly controllable and reproducible optical technique for inducing poration within specific regions of a target cell’s plasma membrane, permitting localized delivery of payloads, depolarization and lysis experiments to be conducted in unprecedented detail. Experiments support a novel mechanism for the process, based upon a thermally-induced change triggered by the interactions of a near-IR laser with a biocompatible thin film substrate at powers substantially below that used in standard optoporation experiments.
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Uncontrolled Keywords: | Cell analysis; Thin films |
| Subjects: | Q Science > QH Natural history > QH301 Biology R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry |
| Divisions: | Civil Engineering & Built Environment |
| Publisher: | Optical Society of America |
| Related URLs: | |
| Date Deposited: | 03 Jun 2015 08:46 |
| Last Modified: | 13 Apr 2022 15:13 |
| DOI or ID number: | 10.1364/BODA.2015.BW1A.5 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/1128 |
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