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Berberine mitigates methotrexate-induced oxidative stress and inflammation in the cerebrum of rats

Mahmoud, AM, Fadel, A, Ramadan, SM and Hozayen, WG (2017) Berberine mitigates methotrexate-induced oxidative stress and inflammation in the cerebrum of rats. Journal of Applied Pharmaceutical Science, 7 (11). pp. 43-49. ISSN 2231-3354

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Abstract

Berberine is a natural isoquinoline alkaloid with multiple beneficial therapeutic effects. This study was designed to evaluate the protective effect of berberine against methotrexate (MTX)-induced oxidative stress and inflammation in the brain of rats. Rats received a single intraperitoneal injection of MTX (20 mg/kg) and orally administered 25 mg/kg and 50 mg/kg body weight berberine for 7 days. MTX-induced rats showed significantly increased lipid peroxidation and nitric oxide levels in the cerebrum. Treatment of the MTXinduced rats with berberine produced a significant decrease in cerebral levels of lipid peroxidation and nitric
oxide. In addition, berberine induced a significant increase in reduced glutathione, superoxide dismutase, glutathione peroxidase and glutathione-S-transferase in the cerebrum of MTX-induced rats. Rats received MTX showed a significant up-regulation of nuclear factor-kappaB (NF-κB), inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNF-α) and interleukin-1beta (IL-1β) expression in the cerebrum, an effect that was significantly reversed following treatment with berberine. In conclusion, berberine protects against MTXinduced neurotoxicity through attenuating oxidative stress and inflammation, and boosting the antioxidant
defenses.

Item Type: Article
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Sports & Exercise Sciences
Publisher: Journal of Applied Pharmaceutical Science
Date Deposited: 03 Oct 2019 08:31
Last Modified: 03 Oct 2019 08:45
DOI or Identification number: 10.7324/JAPS.2017.71106
URI: http://researchonline.ljmu.ac.uk/id/eprint/11425

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