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Genetic Diversity and Gene Family Expansions in Members of the Genus Entamoeba

Wilson, IW, Weedall, GD, Lorenzi, H, Howcroft, T, Hon, C-C, Deloger, M, Guillen, N, Paterson, S, Clark, CG and Hall, N (2019) Genetic Diversity and Gene Family Expansions in Members of the Genus Entamoeba. Genome Biology and Evolution, 11 (3). pp. 688-705. ISSN 1759-6653

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Abstract

Amoebiasis is the third-most common cause ofmortalityworldwide froma parasitic disease.Although the primary etiological agent of amoebiasis is the obligatehuman parasite Entamoeba histolytica, othermembers of the genus Entamoeba can infecthumans and may be pathogenic. Here, we present the first annotated reference genome for Entamoeba moshkovskii, a species that has been associated with human infections, and compare the genomes of E. moshkovskii, E. histolytica, the human commensal Entamoeba dispar, and the nonhuman pathogen Entamoeba invadens. Gene clustering and phylogenetic analyses show differences in expansion and contraction of families of proteins associated with host or bacterial interactions. They intimate the importance to parasitic Entamoeba species of surface-bound proteins involved in adhesion to extracellular membranes, such as the Gal/GalNAc lectin and members of the BspAandAriel1 families. Furthermore, E. dispar is the only one of the four species to lack a functional copy of the key virulence factor cysteine protease CP-A5, whereas the gene’s presence in E. moshkovskii is consistent with the species’ potentially pathogenicnature. Entamoebamoshkovskiiwas foundtobemore diverse thanE.histolytica across all sequence classes. The former is 200 timesmore diverse than latter,with the four E.moshkovskii strains tested having a most recent common ancestor nearly 500 timesmore ancient than the tested E. histolytica strains. A four-haplotype test indicates that these E.moshkovskii strains are not the same species and should be regarded as a species complex.

Item Type: Article
Uncontrolled Keywords: 0604 Genetics, 0603 Evolutionary Biology, 0601 Biochemistry and Cell Biology
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions: Biological & Environmental Sciences (new Sep 19)
Publisher: Oxford University Press
Related URLs:
Date Deposited: 04 Oct 2019 10:49
Last Modified: 04 Oct 2019 11:00
DOI or Identification number: 10.1093/gbe/evz009
URI: http://researchonline.ljmu.ac.uk/id/eprint/11468

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