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Interspecific studies of circadian genes period and timeless in Drosophila

Noreen, S, Pegoraro, M, Nouroz, F, Tauber, E and Kyriacou, CP (2018) Interspecific studies of circadian genes period and timeless in Drosophila. Gene, 648. pp. 106-114. ISSN 0378-1119

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Open Access URL: https://doi.org/10.1016/j.gene.2018.01.020 (Published version)

Abstract

The level of rescue of clock function in genetically arrhythmic Drosophila melanogaster hosts using interspecific clock gene transformation was used to study the putative intermolecular coevolution between interacting clock proteins. Among them PER and TIM are the two important negative regulators of the circadian clock feedback loop. We transformed either the D. pseudoobscura per or tim transgenes into the corresponding arrhythmic D. melanogaster mutant (per01 or tim01) and observed >50% rhythmicity but the period of activity rhythm was either longer (D. pseudoobscura-per) or shorter than 24 h (D. pseudoobscura-tim) compared to controls. By introducing both transgenes simultaneously into double mutants, we observed that the period of the activity rhythm was rescued by the pair of hemizygous transgenes (~24 h). These flies also showed a more optimal level of temperature compensation for the period. Under LD 12:12 these flies have a D. pseudoobscura like activity profile with the absence of morning anticipation as well as a very prominent earlier evening peak of activity rhythm. These observation are consistent with the view that TIM and PER form a heterospecific coevolved module at least for the circadian period of activity rhythms. However the strength of rhythmicity was reduced by having both transgenes present, so while evidence for a coevolution between PER and TIM is observed for some characters it is not for others.

Item Type: Article
Uncontrolled Keywords: 0604 Genetics
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions: Biological & Environmental Sciences (new Sep 19)
Publisher: Elsevier BV
Related URLs:
Date Deposited: 07 Oct 2019 10:33
Last Modified: 07 Oct 2019 10:33
DOI or Identification number: 10.1016/j.gene.2018.01.020
URI: http://researchonline.ljmu.ac.uk/id/eprint/11476

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