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2-OHOA supplementation reduced adiposity and improved cardiometabolic risk to a greater extent than n-3 PUFA in obese mice

Redondo, N, Gheorghe, A, Perez De Heredia Benedicte, F, Diaz, LE, Baccan, GC, de la Fuente, M and Marcos, A 2-OHOA supplementation reduced adiposity and improved cardiometabolic risk to a greater extent than n-3 PUFA in obese mice. Obesity Research and Clinical Practice. ISSN 1871-403X (Accepted)

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Abstract

Objective: We aimed to assess whether 2-hydroxyoleic acid (2-OHOA) and n-3 polyunsaturated fatty acids (PUFA) could counteract changes on adipokine secretion and cardiometabolic risk biomarkers associated with high-fat diet-induced obesity in mice. Methods: Female ICR/CD1 mice (8 weeks old) were divided into four groups receiving different diets (n=8/group): 1) standard chow (control) for 18 weeks; 2) 22% fat for 4 weeks + 60% fat for 14 weeks (obesogenic diet, OD); 3) OD + 2-OHOA (1500 mg·kg−1 diet) for the last 6 weeks (OD-HO); and 4) OD + n-3 PUFA (eicosapentaenoic +docosahexaenoic acids, 1500+1500 mg·kg−1 diet) for the last 6 weeks (OD-N3). After 18 weeks, body weight, periovarian visceral fat, heart and liver weights were measured, as well as cardiometabolic parameters (systolic and diastolic blood pressure, blood glucose, insulin, HOMA index, triglycerides, total cholesterol, apolipoproteins A1 and E), plasma adipokines and inflammatory proteins (leptin, adiponectin, plasminogen activator inhibitor 1 [PAI1], soluble E-selectin [sE-selectin], matrix metalloproteinase-9 [MMP-9], fibrinogen, soluble intercellular adhesion molecule [sICAM] and soluble vascular adhesion molecule [sVCAM]), and secretion of pro-inflamatory cytokines and inflammatory biomarkers from periovarian adipocytes. Results: OD mice had greater body and heart weights, and plasma leptin, and lower adiponectin and resistin secretion from adipocytes. Supplementation with 2-OHOA reduced body and heart weights, blood pressure, triglycerides and leptin, and restored adiponectin and resistin secretion, while n-3 PUFA only reduced triglyceride levels (all P<0.05). Conclusion: 2-OHOA supplementation was more effective in reducing adiposity,modulating adipokine secretion and ameliorating cardiometabolic risk than n-3 PUFA.

Item Type: Article
Uncontrolled Keywords: 11 Medical and Health Sciences, 17 Psychology and Cognitive Sciences
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Biological & Environmental Sciences (new Sep 19)
Publisher: Elsevier
Date Deposited: 08 Nov 2019 10:20
Last Modified: 08 Nov 2019 10:30
URI: http://researchonline.ljmu.ac.uk/id/eprint/11679

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