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Ye, B, Wu, Y, Zhai, X, Zhang, R, Wu, J, Zhang, C, Rahman, K, Qin, L, Han, T and Zheng, C (2020) Beneficial Effects of Endophytic Fungi from the Anoectochilus and Ludisia Species on the Growth and Secondary Metabolism of Anoectochilus roxburghii. ACS Omega, 5 (7). pp. 3487-3497. ISSN 2470-1343
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Beneficial Effects of Endophytic Fungi from the Anoectochilus and Ludisia Species on the Growth and Secondary Metabolism of .pdf - Published Version Download (5MB) | Preview |
Abstract
Endophytic fungi possess favorable effects on their host plants, including disease-resistance improvement, secondary metabolite induction, and growth promotion. It is therefore a promising and sustainable strategy to utilize endophytic fungi for the quality improvement of medicinal herbs or important crops. In our study, a collection of 277 strains of endophytic fungi were isolated from Anoectochilus and Ludisia orchids. Two strains J162 and J211 can be symbiotically cocultured with the tissue culture seedlings of Anoectochilus roxburghii, a popular medicinal and edible plant in southern China. Both strains can significantly enhance the biomass of A. roxburghii and induce the biosynthesis and accumulation of its active ingredients, including flavonoids, kinsenoside, and polysaccharides. J162 and J211 were further identified as Chaetomium globosum and Colletotrichum gloeosporioides based on multilocus phylogenetic analysis. Immunocytochemical staining indicated that J162 and J211 mainly colonized the intercellular gap of xylem parenchyma cells of A. roxburghii roots without obvious harm. In addition, quantitative real-time polymerase chain reaction showed that the expression of three growth-related genes, namely, uracil phosphoribosyl transferase, amino acid transmembrane transporter, and maturase K, were significantly altered in A. roxburghii plants when treated with J162 and J211. In conclusion, the two strains are highly beneficial microbial resources for the growth and accumulation of active ingredients of A. roxburghii in agricultural cultivation.
| Item Type: | Article |
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| Uncontrolled Keywords: | Science & Technology; Physical Sciences; Chemistry, Multidisciplinary; Chemistry; CHAETOMIUM-GLOBOSUM; ORCHIDACEAE; ENZYMES; COLONIZATION; ANTIOXIDANT; INFECTION; MAIZE |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology R Medicine > RV Botanic, Thomsonian, and eclectic medicine |
| Divisions: | Pharmacy & Biomolecular Sciences |
| Publisher: | American Chemical Scociety ACS |
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| Date Deposited: | 02 Apr 2020 10:23 |
| Last Modified: | 04 Sep 2021 07:32 |
| DOI or ID number: | 10.1021/acsomega.9b03789 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/12637 |
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