Tortamano, ACAC, Anselmo, GG, Kassa, CT, Godoy-Miranda, B, Pavani, C, Kato, IT, Wainwright, M and Prates, RA (2020) Antimicrobial photodynamic therapy mediated by methylene blue in surfactant vehicle on periodontopathogens. Photodiagnosis and Photodynamic Therapy, 31. ISSN 1873-1597

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Abstract

Background: Periodontal disease (PD) is a chronic inflammatory disease caused by the presence of microbial biofilm. The aim of this study was to evaluate antimicrobial effect of antimicrobial photodynamic therapy (A-PDT) mediated by methylene blue (MB) in monomer form on A. actinomycetemcomitans and P. gingivalis. Methods: A. actinomycetemcomitans ATCC 29523 and P. gingivalis ATCC 33577 were cultured on anaerobic jars at 37 °C for 48 h, and we tested APDT in the presence of 0.25% sodium dodecyl sulfate (SDS) in phosphate-buffered saline (PBS) or in PBS alone. APDT was carried out with 100 μM MB under laser radiation (PhotolaseIII, DMC, Brazil) at ʎ =660 nm and parameters as following (P =100 mW; I =250 mW/cm2, and doses of 15, 45 and 75 J/cm2). Results: Following A-PDT, PBS groups of A. actinomycetemcomitans presented 4 Logs of microbial death after 5 min irradiation. However, there was no bacterial reduction in SDS groups. On the other hand, P. gingivalis was sensitive to APDT in the presence of 0.25% SDS with 2 logs reduction from dark toxicity. Conclusion: The presence of 0.25% SDS can lead to different responses depending on the different microbial species. © 2020 Elsevier B.V.

Item Type:	Article
Uncontrolled Keywords:	1112 Oncology and Carcinogenesis
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisions:	Pharmacy & Biomolecular Sciences
Publisher:	Elsevier
Date Deposited:	26 May 2020 09:27
Last Modified:	04 Sep 2021 07:15
DOI or ID number:	10.1016/j.pdpdt.2020.101784
URI:	https://researchonline.ljmu.ac.uk/id/eprint/12998

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