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Dahl, M, Areta, J, Jeppersen, P, Jesper, B, Egil, J, Thorsten, I-H, Mette, H, Bjørn, S, John, I, Jorgen, W, Kristian, O and Jørgen, J (2020) Co-ingestion of protein and carbohydrate in the early recovery phase improves endurance performance despite like glycogen degradation and AMPK phosphorylation. Journal of Applied Physiology. ISSN 1522-1601
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Co-ingestion of protein and carbohydrate in the early recovery phase improves endurance performance despite like glycogen degradation and AMPK phosphorylation.pdf - Accepted Version Download (783kB) | Preview |
Abstract
The present study compared the effects of post exercise carbohydrate plus protein (CHO+PROT) and carbohydrate (CHO) only supplementation on muscle glycogen metabolism, anabolic cell signalling and subsequent exercise performance. Nine endurance-trained males cycled twice to exhaustion (muscle glycogen decreased from ~495 to ~125 mmol·kg dw-1) and received either CHO only (1.2 g·kg-1·h-1) or CHO+PROT (0.8/0.4 g·kg-1·h-1) during the first 90 min of recovery. Glycogen content was similar before the performance test after 5 h of recovery. Glycogen synthase (GS) fractional activity increased after exhaustive exercise and remained activated 5 h after despite substantial glycogen synthesis (176.1±19.1 and 204.6±27.0 mmol·kg dw-1 in CHO and CHO+PROT, respectively; p=0.15). Phosphorylation of GS at site 3 and site 2+2a remained low during recovery. After the 5 h recovery, cycling time to exhaustion was improved by CHO+PROT supplementation compared to CHO supplementation (54.6±11.0 vs 46.1±9.8 min; p=0.009). After the performance test, muscle glycogen was equally reduced in PRO+CHO and CHO. Akt Ser473 and p70s6k Thr389 phosphorylation was elevated after 5 h of recovery. There were no differences in Akt Ser473, p70s6k Thr389 or TSC2 Thr1462 phosphorylation between treatments. Nitrogen balance was positive in CHO+PROT (19.6±7.6 mg nitrogen·kg-1, p=0.04) and higher than CHO (-10.7±6.3 mg nitrogen·kg-1, p=0.009). Conclusion: CHO+PROT supplementation during exercise recovery improved subsequent endurance performance relative to consuming CHO only. This improved performance after CHO+PROT supplementation could not be accounted for by differences in glycogen metabolism or anabolic cell signaling, but may have been related to differences in nitrogen balance.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 06 Biological Sciences, 11 Medical and Health Sciences |
| Subjects: | T Technology > TX Home economics > TX341 Nutrition. Foods and food supply R Medicine > RC Internal medicine > RC1200 Sports Medicine |
| Divisions: | Sport & Exercise Sciences |
| Publisher: | American Physiological Society |
| Date Deposited: | 26 Jun 2020 10:17 |
| Last Modified: | 04 Sep 2021 07:06 |
| DOI or ID number: | 10.1152/japplphysiol.00817.2019 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/13192 |
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