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Co-ingestion of protein and carbohydrate in the early recovery phase improves endurance performance despite like glycogen degradation and AMPK phosphorylation

Dahl, M, Areta, J, Jeppersen, P, Jesper, B, Egil, J, Thorsten, I-H, Mette, H, Bjørn, S, John, I, Jorgen, W, Kristian, O and Jørgen, J (2020) Co-ingestion of protein and carbohydrate in the early recovery phase improves endurance performance despite like glycogen degradation and AMPK phosphorylation. Journal of Applied Physiology. ISSN 1522-1601

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Abstract

The present study compared the effects of post exercise carbohydrate plus protein (CHO+PROT) and carbohydrate (CHO) only supplementation on muscle glycogen metabolism, anabolic cell signalling and subsequent exercise performance. Nine endurance-trained males cycled twice to exhaustion (muscle glycogen decreased from ~495 to ~125 mmol·kg dw-1) and received either CHO only (1.2 g·kg-1·h-1) or CHO+PROT (0.8/0.4 g·kg-1·h-1) during the first 90 min of recovery. Glycogen content was similar before the performance test after 5 h of recovery. Glycogen synthase (GS) fractional activity increased after exhaustive exercise and remained activated 5 h after despite substantial glycogen synthesis (176.1±19.1 and 204.6±27.0 mmol·kg dw-1 in CHO and CHO+PROT, respectively; p=0.15). Phosphorylation of GS at site 3 and site 2+2a remained low during recovery. After the 5 h recovery, cycling time to exhaustion was improved by CHO+PROT supplementation compared to CHO supplementation (54.6±11.0 vs 46.1±9.8 min; p=0.009). After the performance test, muscle glycogen was equally reduced in PRO+CHO and CHO. Akt Ser473 and p70s6k Thr389 phosphorylation was elevated after 5 h of recovery. There were no differences in Akt Ser473, p70s6k Thr389 or TSC2 Thr1462 phosphorylation between treatments. Nitrogen balance was positive in CHO+PROT (19.6±7.6 mg nitrogen·kg-1, p=0.04) and higher than CHO (-10.7±6.3 mg nitrogen·kg-1, p=0.009). Conclusion: CHO+PROT supplementation during exercise recovery improved subsequent endurance performance relative to consuming CHO only. This improved performance after CHO+PROT supplementation could not be accounted for by differences in glycogen metabolism or anabolic cell signaling, but may have been related to differences in nitrogen balance.

Item Type: Article
Uncontrolled Keywords: 06 Biological Sciences, 11 Medical and Health Sciences
Subjects: T Technology > TX Home economics > TX341 Nutrition. Foods and food supply
R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Sports & Exercise Sciences
Publisher: American Physiological Society
Date Deposited: 26 Jun 2020 10:17
Last Modified: 26 Jun 2020 10:30
DOI or Identification number: 10.1152/japplphysiol.00817.2019
URI: http://researchonline.ljmu.ac.uk/id/eprint/13192

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