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Simple, sensitive and species-specific assays for detecting quagga and zebra mussels (Dreissena rostriformis bugensis and D. polymorpha) using environmental DNA

Blackman, R, Bennucci, M, Donnelly, R, Hänfling, B, Harper, LR, Sellers, G and Lawson-Handley, L (2020) Simple, sensitive and species-specific assays for detecting quagga and zebra mussels (Dreissena rostriformis bugensis and D. polymorpha) using environmental DNA. Management of Biological Invasions, 11 (2). pp. 218-236. ISSN 1989-8649

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Simple, sensitive and species-specific assays for detecting quagga and zebra mussels (Dreissena rostriformis bugensis and D. polymorpha) using environmental DNA.pdf - Published Version
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Abstract

Early detection is paramount for attempts to remove invasive non-native species (INNS). Traditional methods rely on physical sampling and morphological identification, which can be problematic when species are in low densities and/or are cryptic. The use of environmental DNA (eDNA) as a monitoring tool in freshwater systems is becoming increasingly acceptable and widely used for the detection of single species. Here we demonstrate the development and application of standard PCR primers for the detection of two freshwater invasive species which are high priority for monitoring in the UK and elsewhere: the Dreissenid mussels; Dreissena rostriformis bugensis (Andrusov, 1987) and D. polymorpha (Pallas, 1771). We carried out a rigorous validation process for testing the new primers, including DNA detection and degradation experiments in mesocosms, and a field comparison with traditional monitoring protocols. eDNA from single individuals of both mussel species could be detected within four hours of the start of the mesocosm experiment. In field trials, the two mussel species were detected at all sites where the species are known to be present, and eDNA consistently outperformed traditional kick-net sampling for species detection. These results demonstrate the applicability of standard PCR for eDNA detection of freshwater invasive species.

Item Type: Article
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QL Zoology
Divisions: Biological & Environmental Sciences (new Sep 19)
Publisher: Regional Euro-Asian Biological Invasions Centre (REABIC)
Date Deposited: 02 Jul 2020 12:29
Last Modified: 08 Jul 2020 08:00
DOI or Identification number: 10.3391/mbi.2020.11.2.04
URI: http://researchonline.ljmu.ac.uk/id/eprint/13229

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