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Achieving energy balance with a high‐fat meal does not enhance skeletal muscle adaptation and impairs glycemic response in a sleep‐low training model

Areta, JL, Iraki, J, Owens, DJ, Joanisse, S, Philp, A, Morton, JP and Hallén, J (2020) Achieving energy balance with a high‐fat meal does not enhance skeletal muscle adaptation and impairs glycemic response in a sleep‐low training model. Experimental Physiology. ISSN 0958-0670

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Open Access URL: https://doi.org/10.1113/EP088795 (Published version)

Abstract

Training with low carbohydrate availability (LCHO) has shown to acutely enhance endurance training skeletal muscle response, but concomitant energy deficit (ED) in LCHO interventions has represented a confounding factor in past research. This study aimed at determining if achieving energy balance with high‐fat (EB‐HF) acutely enhances the adaptive response in LCHO compared to ED low‐fat (ED‐LF). In a crossover design, nine well‐trained males completed a ‘sleep‐low’ protocol: on day 1 they cycled to deplete muscle glycogen while reaching a set energy expenditure (30 kcal/kg of fat free mass (FFM)). Post‐exercise, low carbohydrate, protein‐matched meals completely (EB‐HF, 30 kcal/kg FFM) or partially (ED‐LF, 9 kcal/kg FFM) replaced the energy expended, with the majority of energy derived from fat in EB‐HF. In the morning of day 2, participants exercised fasted and skeletal muscle and blood samples were collected and a carbohydrate‐protein drink was ingested at 0.5h recovery. Muscle glycogen showed no treatment effect (P < 0.001) and decreased from 350 ±98 and 192 ±94 mmol/kg dry‐mass between rest and 0.5 h recovery. Phosphorylation status mTOR and AMPK pathway proteins showed only time effects. mRNA expression of p53 increased after exercise (P = 0.005) and was higher in ED‐LF at 3.5h compared to EB‐HF (P = 0.027). Plasma glucose and insulin AUC (P < 0.04) and peak values (P≤0.05) were higher in EB‐HF after the recovery drink. Achieving energy balance with a high‐fat meal in a ‘train‐low’ (‘sleep‐low’) model did not enhance markers of skeletal muscle adaptation and impaired glycemia in response to a recovery drink following training in the morning.

Item Type: Article
Uncontrolled Keywords: 0606 Physiology, 1106 Human Movement and Sports Sciences, 1116 Medical Physiology
Subjects: Q Science > QP Physiology
R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Sport & Exercise Sciences
Publisher: Wiley
Date Deposited: 25 Aug 2020 12:52
Last Modified: 15 Sep 2020 14:45
DOI or Identification number: 10.1113/ep088795
URI: http://researchonline.ljmu.ac.uk/id/eprint/13543

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