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The genetic basis of diurnal preference in Drosophila melanogaster.

Pegoraro, M, Flavell, LMM, Menegazzi, P, Colombi, P, Dao, P, Helfrich-Förster, C and Tauber, E (2020) The genetic basis of diurnal preference in Drosophila melanogaster. BMC Genomics, 21 (1). ISSN 1471-2164

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Open Access URL: https://doi.org/10.1186/s12864-020-07020-z (Published version)

Abstract

BACKGROUND: Most animals restrict their activity to a specific part of the day, being diurnal, nocturnal or crepuscular. The genetic basis underlying diurnal preference is largely unknown. Under laboratory conditions, Drosophila melanogaster is crepuscular, showing a bi-modal activity profile. However, a survey of strains derived from wild populations indicated that high variability among individuals exists, including flies that are nocturnal. RESULTS: Using a highly diverse population, we performed an artificial selection experiment, selecting flies with extreme diurnal or nocturnal preference. After 10 generations, we obtained highly diurnal and nocturnal strains. We used whole-genome expression analysis to identify differentially expressed genes in diurnal, nocturnal and crepuscular (control) flies. Other than one circadian clock gene (pdp1), most differentially expressed genes were associated with either clock output (pdf, to) or input (Rh3, Rh2, msn). This finding was congruent with behavioural experiments indicating that both light masking and the circadian pacemaker are involved in driving nocturnality. CONCLUSIONS: Our study demonstrates that genetic variation segregating in wild populations contributes to substantial variation in diurnal preference. We identified candidate genes associated with diurnality/nocturnality, while data emerging from our expression analysis and behavioural experiments suggest that both clock and clock-independent pathways are involved in shaping diurnal preference. The diurnal and nocturnal selection strains provide us with a unique opportunity to understand the genetic architecture of diurnal preference.

Item Type: Article
Uncontrolled Keywords: 06 Biological Sciences, 08 Information and Computing Sciences, 11 Medical and Health Sciences
Subjects: Q Science > QL Zoology
Divisions: Biological & Environmental Sciences (new Sep 19)
Publisher: BioMed Central
Related URLs:
Date Deposited: 10 Sep 2020 12:00
Last Modified: 10 Sep 2020 12:00
DOI or Identification number: 10.1186/s12864-020-07020-z
URI: http://researchonline.ljmu.ac.uk/id/eprint/13625

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