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PGC-1 alpha alternative promoter (Exon 1b) controls augmentation of total PGC-1 alpha gene expression in response to cold water immersion and low glycogen availability

Allan, R, Morton, JP, Close, GL, Drust, B, Gregson, W and Sharples, AP (2020) PGC-1 alpha alternative promoter (Exon 1b) controls augmentation of total PGC-1 alpha gene expression in response to cold water immersion and low glycogen availability. European Journal of Applied Physiology, 120 (11). pp. 2487-2493. ISSN 1439-6319

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Abstract

This investigation sought to determine whether post-exercise cold water immersion and low glycogen availability, separately and in combination, would preferentially activate either the Exon 1a or Exon 1b Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) promoter. Through a reanalysis of sample design, we identified that the systemic cold-induced augmentation of total PGC-1α gene expression observed previously (Allan et al. in J Appl Physiol 123(2):451–459, 2017) was largely a result of increased expression from the alternative promoter (Exon 1b), rather than canonical promoter (Exon 1a). Low glycogen availability in combination with local cooling of the muscle (Allan et al. in Physiol Rep 7(11):e14082, 2019) demonstrated that PGC-1α alternative promoter (Exon 1b) expression continued to rise at 3 h post-exercise in all conditions; whilst, expression from the canonical promoter (Exon 1a) decreased between the same time points (post-exercise–3 h post-exercise). Importantly, this increase in PGC-1α Exon 1b expression was reduced compared to the response of low glycogen or cold water immersion alone, suggesting that the combination of prior low glycogen and CWI post-exercise impaired the response in gene expression versus these conditions individually. Data herein emphasise the influence of post-exercise cooling and low glycogen availability on Exon-specific control of total PGC-1 α gene expression and highlight the need for future research to assess Exon-specific regulation of PGC-1α.

Item Type: Article
Uncontrolled Keywords: 1106 Human Movement and Sports Sciences
Subjects: R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Sport & Exercise Sciences
Publisher: Springer
Related URLs:
Date Deposited: 03 Nov 2020 12:42
Last Modified: 03 Nov 2020 12:45
DOI or Identification number: 10.1007/s00421-020-04467-6
URI: https://researchonline.ljmu.ac.uk/id/eprint/13944

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