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Enhancing the management of blepharitis.

Iwumune, W (2021) Enhancing the management of blepharitis. Other thesis, Liverpool John Moores University.

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Abstract

Blepharitis is a chronic eye infection affecting the eyelid commonly result from exacerbation of normal microbial flora on the skin and occasionally due to meibomian gland dysfunction. Aim: Identifying organisms responsible for blepharitis with a view to enhancing its management. Method: Investigations of these patients were carried out with the use of slit lamp biomicroscopy after recording their habitual visual acuity. Patients were initially screened out at the GP department before eye examination. Samples were collected with swaps and pure strains were isolated. Samples’ DNA were extracted and amplified through polymerase chain reaction before sequencing. Result: Various strain of Staphylococcal organisms and Pseudomonas aeruginosa were the common organisms identified by 16S rRNA sequencing. Susceptibility tests were performed with most of the organisms found to be insensitive to the antibiotics tested. Conclusion: Blepharitis is known as one of the anterior ocular infection often encountered in clinical practice. It has been noted that clinical presentation does not provide enough diagnostic evidence in management thereby prompting the need for further microbial analysis. Proper identification of causative organisms with the help of more sophisticated and enhanced technique of molecular identification is believed to proffer better outcome in clinical practice.

Item Type: Thesis (Other)
Uncontrolled Keywords: Blepharitis; DNA Extraction; Electrophoresis and Sequence; Molecular Identification
Subjects: R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
R Medicine > RE Ophthalmology
R Medicine > RS Pharmacy and materia medica
Divisions: Pharmacy & Biomolecular Sciences
Date Deposited: 25 Feb 2021 09:32
Last Modified: 25 Feb 2021 09:33
DOI or Identification number: 10.24377/LJMU.t.00014507
Supervisors: Nakouti, I, Hobbs, G and Murphy, M
URI: https://researchonline.ljmu.ac.uk/id/eprint/14507

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