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Isolation and Characterisation of Two Quercetin Glucosides with Potent Anti-Reactive Oxygen Species (ROS) Activity and an Olean-12-en Triterpene Glucoside from the Fruit of Abelmoschus esculentus (L.) Moench

Taiwo, BJ, Popoola, TD, van Heerden, FR and Fatokun, AA (2021) Isolation and Characterisation of Two Quercetin Glucosides with Potent Anti-Reactive Oxygen Species (ROS) Activity and an Olean-12-en Triterpene Glucoside from the Fruit of Abelmoschus esculentus (L.) Moench. Chemistry and Biodiversity, 18 (1). ISSN 1612-1872

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Abstract

Abelmoschus esculentus (Okra) is used in the traditional treatment of cancer, hyperlipidaemia and hyperglycaemia. We, therefore, investigated its composition and potential cytotoxic or antioxidant properties that might underlie its phytotherapeutic applications. Its methanolic fruit extract yielded compounds 1, 2 and 3, identified through NMR, UV and MS analyses as olean‐12‐en‐3‐O‐β‐d‐glucopyranoside, isoquercitrin (quercetin glucoside) and 5,7,3′,4′‐tetrahydroxy‐flavonol‐3‐O‐[β‐d‐glucopyranosyl‐(1→6)]‐β‐d‐glucopyranoside (quercetin diglucoside), respectively. Following 48 h exposure, oleanene glucoside was mildly toxic to the HeLa and the MRC5‐SV2 cancer cells, isoquercitrin was not toxic except at 100 μg/ml in HeLa, and quercetin diglucoside elicited no toxicity. In a 2′,7′‐dichlorofluorescein diacetate (DCFDA) assay of intracellular levels of reactive oxygen species (ROS), hydrogen peroxide increased ROS levels, an effect not affected by oleanene glucoside but protected against by isoquercitrin and quercetin diglucoside, with IC50 values, respectively, of 2.7±0.5 μg/ml and 1.9±0.2 μg/ml (3 h post‐treatment) and 2.0±0.8 μg/ml and 1.5±0.4 μg/ml (24 h post‐treatment.) This is the first report of this oleanene skeleton triterpenoid in the plant. The work provides some insight into why the plant is included in remedies for cancers, cardiovascular complications and diabetes, and reveals it as a potential source of novel therapeutics.

Item Type: Article
Additional Information: This is the peer reviewed version of the following article: B. J. Taiwo, T. D. Popoola, F. R. van Heerden, A. A. Fatokun, Chem. Biodiversity 2021, 18, e2000670., which has been published in final form at https://dx.doi.org/10.1002/cbdv.202000670. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions
Uncontrolled Keywords: 0304 Medicinal and Biomolecular Chemistry, 0305 Organic Chemistry
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
R Medicine > RM Therapeutics. Pharmacology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Wiley
Related URLs:
Date Deposited: 26 Feb 2021 09:17
Last Modified: 26 Feb 2021 09:17
DOI or Identification number: 10.1002/cbdv.202000670
URI: https://researchonline.ljmu.ac.uk/id/eprint/14525

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