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Simultaneous determination of six compounds in rat plasma by ultra-performance liquid chromatography with tandem mass spectrometry: Application in the pharmacokinetic study of Qing Gan-Shu Yu-Fang

Jiang, H, Hou, AJ, Zhang, YY, Man, WJ, Yang, L, Meng, YH, Guo, XY, Wang, S, Zhang, JX, Yang, BY, Wang, QH, Chan, K and Kuang, HX (2019) Simultaneous determination of six compounds in rat plasma by ultra-performance liquid chromatography with tandem mass spectrometry: Application in the pharmacokinetic study of Qing Gan-Shu Yu-Fang. World Journal of Traditional Chinese Medicine, 5 (4). pp. 250-259. ISSN 2311-8571

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Abstract

A rapid and high selective ultra-performance liquid chromatography (UPLC) with tandem mass spectrometry method for simultaneous determination of six compounds including albiflorin, paeoniflorin, picroside I, picroside II, saikosaponin A, and saikosaponin D in rat plasma was developed and validated using butyl p-hydroxybenzoate as an internal standard. One-step direct protein precipitation with acetonitrile was used to extract the compounds from the rat plasma samples. Chromatographic separation was achieved using an ACQUITY UPLC BEH C18column (100 mm × 2.1 mm, 1.7 μm) at a flow rate of 0.4 mL/min, using gradient mode containing 0.1% formic acid in water and acetonitrile were used as the Mobile phase A and B. Electrospray ionization in negative ion mode and multiple reaction monitoring were used to identify and quantify active components. Calibration curves showed good linearity (R2> 0.9908) over a wide concentration range for all compounds. The intra- and interday precision (relative standard deviation) ranged 2.4%-7.0% and 2.6%-8.0%, respectively. The accuracy (relative error) was from -13.0% to 13.2% at all quality control levels. The recovery ranged from 81.1% to 92.5%. The validated method was successfully applied to pharmacokinetic study in rats after oral administration of Qing Gan-Shu Yu-Fang. The results show that one can draw a conclusion that these six active ingredients can be quickly absorbed and play a pharmacodynamic role rapidly in vivo. © 2019 World Journal of Traditional Chinese Medicine | Published by Wolters Kluwer ‑ Medknow.

Item Type: Article
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Wolters Kluwer - Medknow
Date Deposited: 16 Mar 2021 12:58
Last Modified: 16 Mar 2021 13:00
DOI or Identification number: 10.4103/wjtcm.wjtcm_21_19
URI: https://researchonline.ljmu.ac.uk/id/eprint/14630

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