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Activation of beta-adrenoceptors mimics preconditioning of rat-isolated atria and ventricles against ischaemic contractile dysfunction

Penson, PE, Ford, WR, Kidd, EJ and Broadley, KJ (2008) Activation of beta-adrenoceptors mimics preconditioning of rat-isolated atria and ventricles against ischaemic contractile dysfunction. Naunyn-Schmiedeberg's Archives of Pharmacology, 378 (6). ISSN 0028-1298

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Abstract

The effects of ischaemia and reoxygenation on cardiac contractile function can be abrogated by ischaemic preconditioning (IPC). We tested whether β-adrenoceptor agonists could mimic IPC and whether IPC was dependent on β-adrenoceptor activation in rat-isolated cardiac tissues. Paced left atria and right ventricular strips were set-up in Krebs solution and isometric developed tension recorded. Ischaemia was simulated by replacing with hypoxic glucose-free Krebs solution for 30 min. IPC and isoprenaline (10−7 M) preconditioning for 10 min were examined. Developed tension post-reoxygenation was expressed as a percentage of the pre-ischaemic baseline. Recovery at 15 min was significantly increased by IPC in atria (47 ± 4.0% vs. 29.3 ± 1.7%, p < 0.05) and ventricles (39.0 ± 5.2% vs. 22.4 ± 2.8%, p < 0.05). At 60 min, isoprenaline-treated atria recovery (75.8 ± 16.6%) was significantly (p < 0.05) greater than controls (47.9 ± 2.3%). Propranolol (10−6 M) abolished both effects. Therefore, both IPC and β-adrenoceptor agonist-induced improvement of contractile recovery was propranolol-sensitive and β-adrenoceptor-mediated.

Item Type: Article
Uncontrolled Keywords: 1115 Pharmacology and Pharmaceutical Sciences, 1116 Medical Physiology
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Springer
Related URLs:
Date Deposited: 25 Mar 2021 12:24
Last Modified: 25 Mar 2021 12:30
DOI or Identification number: 10.1007/s00210-008-0331-6
URI: https://researchonline.ljmu.ac.uk/id/eprint/14675

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