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Shi, C, Wang, X, Xu, J, Zhang, Y, Qi, B and Jun, L (2021) Dissecting the molecular mechanism of russeting in sand pear ( Pyrus pyrifolia Nakai.) by metabolomics, transcriptomics and proteomics. The Plant Journal. ISSN 0960-7412
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Abstract
The brown colouration and rough appearance as russet and semi-russet (partial russet) are features unique to the popular Asian sand pear (Pyrus pyrifolia Nakai.). The degree of russeting is different between different genotypes. Russeting is sensitive to water fluctuations, where excessive rainwater can trigger/elevate its development. However, the molecular mechanism of russeting is currently unclear. Here, we employed multi-omics, i.e., metabolomics, transcriptomics and proteomics and analyzed the effect of different sand pear genotypes and artificial rainfall on russeting of pear fruits. This led to the identification of 79, 64 and 29 differentially produced/expressed metabolites, transcripts and proteins that are involved in the biosynthesis of suberin, phenylpropane, cutin and waxes. Further analysis of these differentially expressed genes and their encoded proteins revealed that four of them exhibited high expression at both transcript and protein levels. Transient expression of one such gene, PbHHT1 (accession number 103966555) that encodes for ω-hydroxypalmitate-O-feruloyl transferase (HHT) in the young green non-russet fruits triggered premature suberization in the russeting pear genotypes. This coincided with the increased production of 16-feruloyloxypalmitic acid, a conjugated compound between phenols and esters during the polymerization for suberin formation. Collectively, our data from the combined three omics demonstrates that russeting in sand pear is a complex process involving the biosynthesis and transport of suberin and many other secondary metabolites.
| Item Type: | Article |
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| Additional Information: | This is the peer reviewed version of the following article: Shi, C.-H., Wang, X.-Q., Xu, J.-F., Zhang, Y.-X., Qi, B. and Jun, L. (2021), Dissecting the molecular mechanism of russeting in sand pear (Pyrus pyrifolia Nakai) by metabolomics, transcriptomics, and proteomics. Plant J., which has been published in final form at https://doi.org/10.1111/tpj.15532. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited |
| Uncontrolled Keywords: | 0601 Biochemistry and Cell Biology, 0607 Plant Biology |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Pharmacy & Biomolecular Sciences |
| Publisher: | Wiley |
| Date Deposited: | 12 Oct 2021 15:39 |
| Last Modified: | 29 Oct 2022 00:50 |
| DOI or ID number: | 10.1111/tpj.15532 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/15633 |
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