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Dissecting the molecular mechanism of russeting in sand pear ( Pyrus pyrifolia Nakai.) by metabolomics, transcriptomics and proteomics

Shi, C, Wang, X, Xu, J, Zhang, Y, Qi, B and Jun, L Dissecting the molecular mechanism of russeting in sand pear ( Pyrus pyrifolia Nakai.) by metabolomics, transcriptomics and proteomics. The Plant Journal. ISSN 0960-7412 (Accepted)

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Abstract

The brown colouration and rough appearance as russet and semi-russet (partial russet) are features unique to the popular Asian sand pear (Pyrus pyrifolia Nakai.). The degree of russeting is different between different genotypes. Russeting is sensitive to water fluctuations, where excessive rainwater can trigger/elevate its development. However, the molecular mechanism of russeting is currently unclear. Here, we employed multi-omics, i.e., metabolomics, transcriptomics and proteomics and analyzed the effect of different sand pear genotypes and artificial rainfall on russeting of pear fruits. This led to the identification of 79, 64 and 29 differentially produced/expressed metabolites, transcripts and proteins that are involved in the biosynthesis of suberin, phenylpropane, cutin and waxes. Further analysis of these differentially expressed genes and their encoded proteins revealed that four of them exhibited high expression at both transcript and protein levels. Transient expression of one such gene, PbHHT1 (accession number 103966555) that encodes for ω-hydroxypalmitate-O-feruloyl transferase (HHT) in the young green non-russet fruits triggered premature suberization in the russeting pear genotypes. This coincided with the increased production of 16-feruloyloxypalmitic acid, a conjugated compound between phenols and esters during the polymerization for suberin formation. Collectively, our data from the combined three omics demonstrates that russeting in sand pear is a complex process involving the biosynthesis and transport of suberin and many other secondary metabolites.

Item Type: Article
Uncontrolled Keywords: 0601 Biochemistry and Cell Biology, 0607 Plant Biology
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Wiley
Date Deposited: 12 Oct 2021 15:39
Last Modified: 12 Oct 2021 15:45
DOI or Identification number: 10.1111/tpj.15532
URI: https://researchonline.ljmu.ac.uk/id/eprint/15633

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