Tools
Tools
Beldjilali-Labro, M, Jellali, R, Brown, AD, Garcia Garcia, A, Lerebours, A, Guenin, E, Bedoui, F, Dufresne, M, Stewart, C, Grosset, J-F and Legallais, C (2021) Multiscale-Engineered Muscle Constructs: PEG Hydrogel Micro-Patterning on an Electrospun PCL Mat Functionalized with Gold Nanoparticles. International Journal of Molecular Sciences, 23 (1). ISSN 1422-0067
|
Text
Multiscale-Engineered Muscle Constructs PEG Hydrogel Micro-Patterning on an Electrospun PCL Mat Functionalized with Gold Nanoparticles.pdf - Published Version Available under License Creative Commons Attribution. Download (4MB) | Preview |
Abstract
The development of new, viable, and functional engineered tissue is a complex and challenging task. Skeletal muscle constructs have specific requirements as cells are sensitive to the stiffness, geometry of the materials, and biological micro-environment. The aim of this study was thus to design and characterize a multi-scale scaffold and to evaluate it regarding the differentiation process of C2C12 skeletal myoblasts. The significance of the work lies in the microfabrication of lines of polyethylene glycol, on poly(ε-caprolactone) nanofiber sheets obtained using the electrospinning process, coated or not with gold nanoparticles to act as a potential substrate for electrical stimulation. The differentiation of C2C12 cells was studied over a period of seven days and quantified through both expression of specific genes, and analysis of the myotubes' alignment and length using confocal microscopy. We demonstrated that our multiscale bio-construct presented tunable mechanical properties and supported the different stages skeletal muscle, as well as improving the parallel orientation of the myotubes with a variation of less than 15°. These scaffolds showed the ability of sustained myogenic differentiation by enhancing the organization of reconstructed skeletal muscle. Moreover, they may be suitable for applications in mechanical and electrical stimulation to mimic the muscle's physiological functions.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 0399 Other Chemical Sciences, 0604 Genetics, 0699 Other Biological Sciences |
| Subjects: | Q Science > QH Natural history > QH426 Genetics R Medicine > RC Internal medicine > RC1200 Sports Medicine |
| Divisions: | Sport & Exercise Sciences |
| Publisher: | MDPI AG |
| Related URLs: | |
| Date Deposited: | 17 Jan 2022 10:16 |
| Last Modified: | 17 Jan 2022 10:30 |
| DOI or ID number: | 10.3390/ijms23010260 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/16068 |
 |
View Item |