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Acute Inflammation and Oxidative Stress Induced by Lipopolysaccharide and the Ameliorative Effect of Stingless Bee Honey

Ranneh, Y, Mahmoud, AM, Fadel, A, Albujja, M, Akim, AM, Ab Hamid, H and Khazaai, H (2020) Acute Inflammation and Oxidative Stress Induced by Lipopolysaccharide and the Ameliorative Effect of Stingless Bee Honey. COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING, 24 (6). pp. 744-757. ISSN 1386-2073

fulltextBMS-CCHTS-2020-HT14-1011-41.pdf - Accepted Version

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Background: Systemic acute inflammation is the hallmark of sepsis and is associated with multiple organ dysfunction.

Objective: This study investigated the potential of Stingless Bee Honey (SBH) to suppress lipopolysaccharide (LPS)-induced systemic acute inflammation in rats and to reveal the probable mechanism of action.

Methods: Rats received 4.6 and 9.2 g/kg SBH for 7 days followed by a single injection of LPS after which blood samples were taken 6h later.

Results: LPS induced liver, kidney, heart, and lung injury, were manifested by increased serum transaminases, alkaline phosphatase, creatine kinase, creatinine, and urea, along with multiple histological alterations, particularly leukocyte infiltration. Pro-inflammatory cytokines were elevated in the serum, and NF-κB p65, p38 MAPK, and HMGB-1 were significantly increased in different tissues of LPS-challenged rats. SBH prevented tissue injury, ameliorated pro-inflammatory cytokines, and suppressed NF-κB p65, p38 MAPK, and HMGB-1 in rats that had received LPS. In addition, SBH diminished reactive oxygen species (ROS) production, lipid peroxidation, and oxidative DNA damage, and enhanced glutathione and Nrf2 in LPS-treated rats.

Conclusion: SBH prevents systemic acute inflammation by suppressing NF-κB, p38 MAPK, HMGB-1, oxidative stress, and tissue injury in rats. Thus, SBH may represent an effective anti-inflammatory nutraceutical, pending further mechanistic studies.

Item Type: Article
Additional Information: The published manuscript is available at EurekaSelect via http://www.eurekaselect.com/10.2174/1386207323999200918152111
Uncontrolled Keywords: 0304 Medicinal and Biomolecular Chemistry, 0601 Biochemistry and Cell Biology
Subjects: Q Science > QD Chemistry
R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Sport & Exercise Sciences
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Date Deposited: 14 Apr 2022 14:47
Last Modified: 14 Apr 2022 15:00
DOI or ID number: 10.2174/1386207323999200918152111
URI: https://researchonline.ljmu.ac.uk/id/eprint/16514
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