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iChip increases the success of cultivation of TBT-resistant and TBT-degrading bacteria from estuarine sediment

Polrot, A, Kirby, JR, Olorunniji, FJ, Birkett, JW and Sharples, GP (2022) iChip increases the success of cultivation of TBT-resistant and TBT-degrading bacteria from estuarine sediment. World Journal of Microbiology and Biotechnology, 38 (10). ISSN 0959-3993

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Standard methods of microbial cultivation only enable the isolation of a fraction of the total environmental bacteria. Numerous techniques have been developed to increase the success of isolation and cultivation in the laboratory, some of which derive from diffusion chambers. In a diffusion chamber, environmental bacteria in agar medium are put back in the environment to grow as close to their natural conditions as possible, only separated from the environment by semi-permeable membranes. In this study, the iChip, a device that possesses hundreds of mini diffusion chambers, was used to isolate tributyltin (TBT) resistant and degrading bacteria. IChip was shown to be efficient at increasing the number of cultivable bacteria compared to standard methods. TBT-resistant strains belonging to Oceanisphaera sp., Pseudomonas sp., Bacillus sp. and Shewanella sp. were identified from Liverpool Dock sediment. Among the isolates in the present study, only members of Pseudomonas sp. were able to use TBT as a sole carbon source. It is the first time that members of the genus Oceanisphaera have been shown to be TBT-resistant. Although iChip has been used in the search for molecules of biomedical interest here we demonstrate its promising application in bioremediation.

Item Type: Article
Uncontrolled Keywords: 06 Biological Sciences; 10 Technology; Biotechnology
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QH Natural history > QH301 Biology
R Medicine > RS Pharmacy and materia medica
T Technology > T Technology (General)
Divisions: Biological & Environmental Sciences (from Sep 19)
Pharmacy & Biomolecular Sciences
Publisher: Springer
SWORD Depositor: A Symplectic
Date Deposited: 17 Aug 2022 09:11
Last Modified: 17 Aug 2022 09:15
DOI or ID number: 10.1007/s11274-022-03297-2
URI: https://researchonline.ljmu.ac.uk/id/eprint/17407
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