Viteri, M, Fiebich, B, Prieto Garcia, J and Heinrich, M (2022) Effects of important plant species of Teruel on the pro-inflamatory signalling cascade of human monocytes. Journal of Natural Products Discovery, 1 (3). ISSN 2755-1997

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Abstract

Background: The study of medicinal and edible plants from Teruel (Spain) –one of the biodiverse regions of the Mediterranean has been particularly limited tothe write-up of taxonomical databases and lists of protected species. The following analysis and work will be the first for this region and will focus on the anti-inflammatory activity of plant species, based on information gained through knowledge oftheir traditional use. Aims: Touse a multitarget, in vitroapproach focusing on the antioxidant and pro-inflammatory signalling cascade. To provide a phytochemical basis to the traditional and oral traditions of ethnographic research. Methods: Plants extracts (1-50 μg/mL)from Teruel recorded as traditionallyusedagainst indicationsrelated to inflammation were assessed as potential inhibitors of the release of inflammatory mediators namely interleukin (IL)-6, IL-8, tumour necrosis factor (TNF)-alpha) and prostaglandin (PG) E2 in primary human monocytes challenged with 10 ng/mL of LPS. Their antioxidant activity as the inhibition of thiobarbituric reactive substances (TBARS), total phenolic content(Folin-Ciocalteau)and phenolic fingerprint (HPLC-UV-DAD)were also evaluated.Results: Berberis vulgaris, Jasonia glutinosa, Satureja montana andSideritis hirsuta ethanol extracts were potent inhibitors of cytokine production but did not exert significant effects on prostaglandins synthesis. This effect was even stronger when monocytes were incubated in the presence of both aqueous and ethanol extracts of Thymus vulgaris. Prunus spinosaethanol extract presents an overall strong pro-inflammatory effect instead. B.vulgaris, J.glutinosa, S.montana andT.vulgarisethanol extracts were the richest in phenols (c.a.2 mg/mL caffeic acid equivalents) but the antioxidant effect did not correlate with this parameter pointing towards the presence of potent antioxidants from other phytochemical classes.Conclusion: Overall, this project provides evidence of the efficacy attributed to severalspecies central to the medical folklore in Teruel (Spain). Their anti-inflammatory and antioxidant activity profile will support further phytochemical-pharmacological investigations.

Item Type:	Article
Uncontrolled Keywords:	Ethnopharmacology; Phenols; Antioxidant; Anti-inflammatory
Subjects:	R Medicine > RS Pharmacy and materia medica R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions:	Pharmacy & Biomolecular Sciences
Publisher:	LJMU
SWORD Depositor:	A Symplectic
Date Deposited:	17 Jan 2023 12:47
Last Modified:	17 Jan 2023 12:47
DOI or ID number:	10.24377/jnpd.article700
Editors:	Sarker, Satya and Fielding, Alistair
URI:	https://researchonline.ljmu.ac.uk/id/eprint/18658

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