Okoye, JC, Holland, A, Pitoulias, M, Paschalis, V, Piddubnyi, A, Dufailu, OA, Borén, T, Oldfield, NJ, Mahdavi, J and Soultanas, P (2023) Ferric quinate (QPLEX) inhibits the interaction of major outer membrane protein (MOMP) with the Lewis b (Leb) antigen and limits Campylobacter colonization in broilers. Frontiers in Microbiology, 14.

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Abstract

Campylobacter jejuni colonizes hosts by interacting with Blood Group Antigens (BgAgs) on the surface of gastrointestinal epithelia. Genetic variations in BgAg expression affects host susceptibility to C. jejuni. Here, we show that the essential major outer membrane protein (MOMP) of C. jejuni NCTC11168 binds to the Lewis b (Leb) antigen on the gastrointestinal epithelia of host tissues and this interaction can be competitively inhibited by ferric quinate (QPLEX), a ferric chelate structurally similar to bacterial siderophores. We provide evidence that QPLEX competitively inhibits the MOMP-Leb interaction. Furthermore, we demonstrate that QPLEX can be used as a feed additive in broiler farming to significantly reduce C. jejuni colonization. Our results indicate that QPLEX can be a viable alternative to the preventative use of antibiotics in broiler farming to combat C. jejuni infections.

Item Type:	Article
Uncontrolled Keywords:	0502 Environmental Science and Management; 0503 Soil Sciences; 0605 Microbiology
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisions:	Pharmacy & Biomolecular Sciences
Publisher:	Frontiers Media SA
SWORD Depositor:	A Symplectic
Date Deposited:	29 Jun 2023 11:07
Last Modified:	29 Jun 2023 11:07
DOI or ID number:	10.3389/fmicb.2023.1146418
URI:	https://researchonline.ljmu.ac.uk/id/eprint/20132

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