Srisawat, K, Stead, CA, Hesketh, K, Pogson, MA, Strauss, JA, Cocks, MS, Siekmann, I, Phillips, SM, Lisboa, PJ, Shepherd, SO and Burniston, JG (2023) People with obesity exhibit losses in muscle proteostasis that are partly improved by exercise training. Proteomics. pp. 1-14. ISSN 1615-9853

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Abstract

This pilot experiment examines if a loss in muscle proteostasis occurs in people with obesity and whether endurance exercise positively influences either the abundance profile or turnover rate of proteins in this population. Men with (n = 3) or without (n = 4) obesity were recruited and underwent a 14-d measurement protocol of daily deuterium oxide (D2 O) consumption and serial biopsies of vastus lateralis muscle. Men with obesity then completed 10-weeks of high-intensity interval training (HIIT), encompassing 3 sessions per week of cycle ergometer exercise with 1 min intervals at 100% maximum aerobic power interspersed by 1 min recovery periods. The number of intervals per session progressed from 4 to 8, and during weeks 8-10 the 14-d measurement protocol was repeated. Proteomic analysis detected 352 differences (p < 0.05, false discovery rate < 5%) in protein abundance and 19 (p < 0.05) differences in protein turnover, including components of the ubiquitin-proteasome system. HIIT altered the abundance of 53 proteins and increased the turnover rate of 22 proteins (p < 0.05) and tended to benefit proteostasis by increasing muscle protein turnover rates. Obesity and insulin resistance are associated with compromised muscle proteostasis, which may be partially restored by endurance exercise.

Item Type:	Article
Uncontrolled Keywords:	biosynthetic labelling; deuterium oxide; fractional synthesis rate; heat shock proteins; heavy water; muscle protein synthesis; protein turnover; proteomics; skeletal muscle; ubiquitin proteasome system; 06 Biological Sciences; 08 Information and Computing Sciences; 11 Medical and Health Sciences; Biochemistry & Molecular Biology
Subjects:	R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions:	Computer Science & Mathematics Sport & Exercise Sciences
Publisher:	Wiley
SWORD Depositor:	A Symplectic
Date Deposited:	20 Nov 2023 10:50
Last Modified:	20 Nov 2023 11:00
DOI or ID number:	10.1002/pmic.202300395
URI:	https://researchonline.ljmu.ac.uk/id/eprint/21904

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