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Mutations of voltage-gated sodium channel contribute to pyrethroid resistance in Panonychus citri

Pan, D, Luo, Q-J, O Reilly, AO, Yuan, G-R, Wang, J-J and Dou, W (2023) Mutations of voltage-gated sodium channel contribute to pyrethroid resistance in Panonychus citri. Insect science. pp. 1-14. ISSN 1672-9609

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Insecticide resistance in Panonychus citri is a major obstacle to mite control in citrus orchards. Pyrethroid insecticides are continually used to control mites in China, although resistance to pyrethroids has evolved in some populations. Here, the resistance to the pyrethroid fenpropathrin was investigated and 7 out of 8 field-collected populations of P. citri exhibited a high level of resistance, ranging from 171-fold to 15 391-fold higher than the susceptible (SS) comparison strain. Three voltage-gated sodium channel (VGSC) mutations were identified in the tested populations: L1031V, F1747L, and F1751I. Amplicon sequencing was used to evaluate the frequency of these mutations in the 19 field populations. L1031V and F1751I were present in all populations at frequencies of 11.6%-82.1% and 0.5%-31.8%, respectively, whereas the F1747L mutation was only present in 12 populations from Chongqing, Sichuan, Guangxi, and Yunnan provinces. Introduction of these mutations singly or in combination into transgenic flies significantly increased their resistance to fenpropathrin and these flies also exhibited reduced mortality after exposure to the pyrethroids permethrin and β-cypermethrin. Panonychus citri VGSC homology modeling and ligand docking indicate that F1747 and F1751 form direct binding contacts with pyrethroids, which are lost with mutation, whereas L1031 mutation may diminish pyrethroid effects through an allosteric mechanism. Overall, the results provide molecular markers for monitoring pest resistance to pyrethroids and offer new insights into the basis of pyrethroid actions on sodium channels.

Item Type: Article
Additional Information: This is the peer reviewed version of the following article: Pan, D., Luo, Q.-J., O´Reilly, A.O., Yuan, G.-R., Wang, J.-J. and Dou, W. (2023), Mutations of voltage-gated sodium channel contribute to pyrethroid resistance in Panonychus citri. Insect Science. https://doi.org/10.1111/1744-7917.13266, which has been published in final form at https://doi.org/10.1111/1744-7917.13266. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
Uncontrolled Keywords: Panonychus citri; amplicon sequencing; kdr mutation; molecular docking; pyrethroid resistance; transgenic flies; 0501 Ecological Applications; 0608 Zoology; Entomology
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QH Natural history > QH301 Biology
Q Science > QL Zoology
Divisions: Biological & Environmental Sciences (from Sep 19)
Publisher: Wiley
SWORD Depositor: A Symplectic
Date Deposited: 18 Jan 2024 09:30
Last Modified: 18 Jan 2024 09:30
DOI or ID number: 10.1111/1744-7917.13266
URI: https://researchonline.ljmu.ac.uk/id/eprint/22309
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