Habibi, E, Sepehrara, A, Arabnozari, H, Sharifianzaji, F, Enderami, SE, Sarker, SD, Hassannia, H and Nahar, L (2024) Comparative evaluation of the anti-proliferative effects of alkaloid-rich extract of jujube seed and paclitaxel on MDA-MB-231 breast cancer cell line. Journal of Agriculture and Food Research, 18. ISSN 2666-1543

Preview

Text Comparative evaluation of the anti-proliferative effects of alkaloid rich extract of jujube seed.pdf - Published Version Available under License Creative Commons Attribution Non-commercial. Download (2MB) | Preview

Abstract

Introduction: Cancer is the second cause of death in the world, and among all types of cancer, triple-negative breast cancer (TNBC) has one of the worst prognoses. Repeated use of chemotherapy drugs is associated with drug resistance. This study aims to investigate the effect of jujube alkaloid-rich extract on drug sensitivity and its anti-tumor effects on paclitaxel-resistant MDA-MB-231 cells in vitro. Materials and methods: The MDA-MB-231 breast cancer cell line and its paclitaxel-resistant variant were used. The XTT method was employed to measure cell viability, while Annexin-PI assays were used to detect apoptosis. Various concentrations of the jujube extract alone and in combination with paclitaxel assessed synergistic effects in 2D and 3D cell culture models. Results: The presence of alkaloids in the extract obtained from the jujube seed was confirmed by the dragendorff test and its amount was 0.395 g in 5.0 g of jujube seed powder. Paclitaxel and jujube extract exhibited dose-dependent cytotoxic effects on paclitaxel-resistant MDA-MB-231 cells. The combination of jujube extract and paclitaxel significantly decreased IC50 from 541.3 μg/mL to 124.3 μg/mL, and the IC20 decreased from 92.1 μg/mL to 23.9 μg/mL when combined with paclitaxel in 2D culture model. The combination's toxicity was reduced in the 3D culture model. Apoptosis rates were 0.75 % in the control group, 13.89 % in the alkaloid-rich extract group, 2.59 % in the paclitaxel group, and 42.90 % in the combination group. Conclusion: Combining paclitaxel with alkaloid-rich jujube seed extract enhances cytotoxicity and apoptosis in paclitaxel-resistant MDA-MB-231 breast cancer cells in both 2D and 3D culture models. This suggests that such combinations might be a viable strategy to overcome drug resistance in TNBC treatments.

Item Type:	Article
Subjects:	R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica
Divisions:	Pharmacy and Biomolecular Sciences
Publisher:	Elsevier
SWORD Depositor:	A Symplectic
Date Deposited:	07 Oct 2024 13:40
Last Modified:	07 Oct 2024 13:45
DOI or ID number:	10.1016/j.jafr.2024.101438
URI:	https://researchonline.ljmu.ac.uk/id/eprint/24423

View Item