Tools
Tools
Zareena, B, Jeelani, S.U.Y, Khadim, A, Ali, A, Uddin, J, Sarker, SD, Rainer, M, Khalifa, S.A.M, El-Seedi, H.R, Ramzan, M and Musharraf, SG (2024) Development of a Tandem Mass Spectral Library for the Detection of Triterpenoids in Plant Metabolome Based on Reference Standards. Plants, 13 (23). ISSN 2223-7747
|
Text
Plants2024.pdf - Published Version Available under License Creative Commons Attribution. Download (1MB) | Preview |
Abstract
Plant triterpenoids represent a diverse group of secondary metabolites and are thought to be valuable for therapeutic applications. For drug development, lead optimization, better knowledge of biological pathways, and high-throughput detection of secondary metabolites in plant extracts are crucial. This paper describes a qualitative method for the rapid and accurate identification of various triterpenoids in plant extracts using the LC-HR-ESI-MS/MS tool in combination with the data-dependent acquisition (DD) approach. A total of 44 isolated, purified, and characterized triterpenoids were analyzed. HR-MS spectra and tandem mass spectra (MS/MS) of each compound were recorded in the positive ionization mode in two different sets of collisional energies, i.e., (25–62.5 eV), and fixed collisional energies (10, 20, 30, and 40 eV). As a result, three triterpenoids were identified in all plant extracts using the retention time, high-resolution mass spectra, and/or MS/MS spectra. The developed method will be helpful with other plant extracts/botanicals, as well as in the search for new triterpenoids in the kingdom Plantae.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica R Medicine > RV Botanic, Thomsonian, and eclectic medicine |
| Divisions: | Pharmacy and Biomolecular Sciences |
| Publisher: | MDPI |
| Related URLs: | |
| SWORD Depositor: | A Symplectic |
| Date Deposited: | 22 Nov 2024 11:53 |
| Last Modified: | 22 Nov 2024 12:00 |
| DOI or ID number: | 10.3390/plants13233278 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/24842 |
 |
View Item |