Iqbal, AMA, Sarkar, A, Saha, A, Bhardwaj, P, Panchadhavee, R, Syveena, S, Somabattini, R, Das, N, Nanjappan, SK, Unger, BS, Hazra, A, Ghosh, P, Babykutty, S, Nahar, L, Sarker, SD, Palit, P and Chattopadhyay, D Phytochemical, LC-MS Studies, and Assessment of Antioxidant , Anti-inflammatory,hypoglycemic, anti-Rheumatoid-arthritis potential with Acmella paniculata : in-silico and in-vitro appraisal. ChemistrySelect. ISSN 2365-6549 (Accepted)

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Abstract

Acmella paniculata (AP), an ethnomedicinal plant, is traditionally used for toothache, pain, inflammation, and arthritis, as edible tribal vegetables and as an antimicrobial agent. This study aimed to evaluate the protective role of AP against inflammatory pain, rheumatoid arthritis (RA), and hyperglycemia. Hydro-ethanolic flower extract of AP (HEFeAP) was prepared via maceration, followed by ultrasonication. Physicochemical characterization was performed using Thin Layer Chromatography (TLC), Liquid Chromatography-Mass Spectrometry (LC-MS), Ultra-violet Spectroscopy (UV), and Fourier Transform Infrared Spectroscopy (FTIR). Major phytochemicals were assessed against inflammatory mediators using molecular docking and Molecular Dynamics (MD) simulations. HEFeAP’s antioxidant activity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) (31.22±0.53 µg/ml) and hydrogen peroxide (H2O2) scavenging (40.36±0.47 µg/ml). The anti-inflammatory activity, protective role against RA, and anti-hyperglycaemic activities were evaluated via egg-albumin denaturation, MTT assay against human-fibroblast cells and α-amylase inhibition, showing IC50 values of 27.61, 65.75, and 85.43 µg/ml, respectively. Tumor Necrosis Factor-alpha (TNF-α), and nitric-oxide (NO) levels were significantly downregulated in LPS-stimulated PBMC-derived macrophages (IC50 = 152 and 164.56 µg/ml). Phytochemical analysis revealed coumarins, flavonoids, polyphenols, steroids, and local-anesthetic alkylamides. Active phytomolecules in HEFeAP exhibited strong and stable binding with inflammatory biomarkers. These findings suggest HEFeAP can be used as a potential candidate for treating inflammatory pain, Rheumatoid Arthritis (RA), and diabetes.

Item Type:	Article
Uncontrolled Keywords:	03 Chemical Sciences; 34 Chemical sciences
Subjects:	R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica
Divisions:	Pharmacy and Biomolecular Sciences
Publisher:	Wiley
SWORD Depositor:	A Symplectic
Date Deposited:	10 Apr 2025 15:19
Last Modified:	10 Apr 2025 15:19
URI:	https://researchonline.ljmu.ac.uk/id/eprint/26165

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