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Influence of sex, age, pubertal maturation and body mass index on circulating white blood cell counts in healthy European adolescents—the HELENA study

Pérez-de-Heredia, F, Gómez-Martínez, S, Díaz, L-E, Veses, AM, Nova, E, Marcos, A, Wärnberg, J, Wastlund, A, Sjöstrom, M, Huybrechts, I, Vyncke, K, Androutsos, O, Manios, Y, Ferrari, M, Palacios, G, González-Gross, M, Kovács, É, Molnár, D, Gottrand, F, Castillo, MJ , Kafatos, A, Widhalm, K and Moreno, LA (2015) Influence of sex, age, pubertal maturation and body mass index on circulating white blood cell counts in healthy European adolescents—the HELENA study. European Journal of Pediatrics, 174 (8). pp. 999-1014. ISSN 0340-6199

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Percentiles 10th, 25th, 50th, 75th and 90th are presented for circulating white blood cells (WBC), neutrophils, lymphocytes, monocytes, eosinophils and basophils in healthy European adolescents (12.5–17.5 years, n = 405, 48.9 % boys), considering age, sex, puberty and body mass index (BMI). CD3<sup>+</sup> (mature T cells), CD4<sup>+</sup> (T helper), CD8<sup>+</sup> (T cytotoxic), CD16<sup>+</sup>56<sup>+</sup> (natural killer), CD19<sup>+</sup> (B cells), CD3<sup>+</sup>CD45RA<sup>+</sup>, CD4<sup>+</sup>CD45RA<sup>+</sup>, CD8<sup>+</sup>CD45RA<sup>+</sup> (naïve), CD3<sup>+</sup>CD45RO<sup>+</sup>, CD4<sup>+</sup>CD45RO<sup>+</sup> and CD8<sup>+</sup>CD45RO<sup>+</sup> (memory) lymphocytes were also analysed by immunophenotyping. Girls presented higher WBC, neutrophil, CD3<sup>+</sup>CD45RO<sup>+</sup> and CD4<sup>+</sup>CD45RO<sup>+</sup> cell counts and CD3<sup>+</sup>/CD19<sup>+</sup> ratio, and lower CD3<sup>+</sup>CD45RA<sup>+</sup> and CD4<sup>+</sup>CD45RA<sup>+</sup> counts than boys. Age was associated with higher neutrophil counts and CD3<sup>+</sup>/CD19<sup>+</sup>, and lower CD19<sup>+</sup> counts; in boys, with lower CD3<sup>+</sup>CD45RA<sup>+</sup>, CD4<sup>+</sup>CD45RA<sup>+</sup> and CD8<sup>+</sup>CD45RA<sup>+</sup> counts as well; in girls, with higher WBC, CD3<sup>+</sup>CD45RO<sup>+</sup> and CD4<sup>+</sup>CD45RO<sup>+</sup> counts. Pubertal maturation in boys was associated with lower WBC and lymphocyte counts; in girls, with higher basophil, CD3<sup>+</sup>CD45RO<sup>+</sup> and CD4<sup>+</sup>CD45RO<sup>+</sup> values. BMI was associated with higher WBC counts; in boys, also with higher lymphocyte counts; in girls, with higher neutrophil, CD4<sup>+</sup>, CD3<sup>+</sup>CD45RO<sup>+</sup> and CD4<sup>+</sup>CD45RO<sup>+</sup> counts. Conclusion: Our study provides normative values for circulating immune cells in adolescents, highlighting the importance of considering sex, age, pubertal maturation and BMI when establishing reference ranges for WBC in paediatric populations.

Item Type: Article
Additional Information: The final publication is available at Springer via http://dx.doi.org/10.1007/s00431-015-2497-5
Uncontrolled Keywords: 1114 Paediatrics And Reproductive Medicine, 1117 Public Health And Health Services
Subjects: R Medicine > RJ Pediatrics
R Medicine > RJ Pediatrics > RJ101 Child Health. Child health services
Divisions: Natural Sciences & Psychology (closed 31 Aug 19)
Publisher: Springer
Date Deposited: 17 Feb 2016 09:40
Last Modified: 23 May 2022 14:47
DOI or ID number: 10.1007/s00431-015-2497-5
URI: https://researchonline.ljmu.ac.uk/id/eprint/2938
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