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Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture

Storm, MP, Sorrell, I, Shipley, R, Regan, S, Luetchford, KA, Sathish, J, Webb, SD and Ellis, MJ (2016) Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture. Journal of Visualized Experiments, 111 (e53431). pp. 1-12. ISSN 1940-087X

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Tissue culture has been used for over 100 years to study cells and responses ex vivo. The convention of this technique is the growth of anchorage dependent cells on the 2-dimensional surface of tissue culture plastic. More recently, there is a growing body of data demonstrating more in vivo-like behaviors of cells grown in 3-dimensional culture systems. This manuscript describes in detail the set-up and operation of a hollow fiber bioreactor system for the in vivo-like culture of mammalian cells. The hollow fiber bioreactor system delivers media to the cells in a manner akin to the delivery of blood through the capillary networks in vivo. The system is designed to fit onto the shelf of a standard CO2 incubator and is simple enough to be set-up by any competent cell biologist with a good understanding of aseptic technique. The systems utility is demonstrated by culturing the hepatocarcinoma cell line HepG2/C3A for 7 days. Further to this and in line with other published reports on the functionality of cells grown in 3-dimensional culture systems the cells are shown to possess increased albumin production (an important hepatic function) when compared to standard 2-dimensional tissue culture.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Applied Mathematics (merged with Comp Sci 10 Aug 20)
Publisher: Journal of Visualized Experiments
Date Deposited: 10 Jun 2016 11:36
Last Modified: 02 Mar 2022 10:06
DOI or ID number: 10.3791/53431
URI: https://researchonline.ljmu.ac.uk/id/eprint/3753
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