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Rubisco catalytic properties and temperature response in crops.

Hermida-Carrera, C, Kapralov, MV and Galmés, J (2016) Rubisco catalytic properties and temperature response in crops. Plant Physiol. ISSN 0032-0889

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Rubisco catalytic traits and their thermal dependence are two major factors limiting the CO2 assimilation potential of plants. In this study, we present the profile of Rubisco kinetics for twenty crop species at three different temperatures. The results largely confirmed the existence of significant variation in the Rubisco kinetics among species. Although some of the species tended to present Rubisco with higher thermal sensitivity (e.g., Oryza sativa) than others (e.g., Lactuca sativa), interspecific differences depended on the kinetic parameter. Comparing the temperature response of the different kinetic parameters, the Rubisco Michaelis-Menten constants for CO2 (Kc and Kcair) presented higher energy of activation (ΔHa) than the maximum carboxylation rate (kcatc) and the CO2 compensation point in the absence of mitochondrial respiration (Γ*). The analysis of the Rubisco large subunit sequence revealed the existence of some sites under adaptive evolution in branches with specific kinetic traits. Because Rubisco kinetics and their temperature dependency were species-specific, they largely affected the assimilation potential of Rubisco from the different crops, especially under those conditions (i.e., low CO2 availability at the site of carboxylation and high temperature) inducing Rubisco-limited photosynthesis. As an example, at 25 ºC, Rubisco from Hordeum vulgare and Glycine max presented, respectively, the highest and lowest potential for CO2 assimilation at both high and low chloroplastic CO2 concentrations. In our opinion, this information is relevant to improve photosynthesis models and should be considered in future attempts to design more efficient Rubiscos.

Item Type: Article
Additional Information: This s an accepted version of an article published in Plant Physiology 21/06/2016 http:/​/​dx.​doi.​org/​10.​1104/​pp.​16.​01846
Uncontrolled Keywords: 06 Biological Sciences, 07 Agricultural And Veterinary Sciences
Subjects: Q Science > QK Botany
S Agriculture > S Agriculture (General)
Divisions: Natural Sciences & Psychology (closed 31 Aug 19)
Publisher: Amercian society of plant biologists
Related URLs:
Date Deposited: 08 Jul 2016 09:58
Last Modified: 04 Sep 2021 12:44
DOI or ID number: 10.1104/pp.16.01846
URI: https://researchonline.ljmu.ac.uk/id/eprint/3863
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