Tools
Tools
Mohammed, T, Murphy, MF, Lilley, F, Burton, DR and Bezombes, F (2016) The effects of acoustic vibration on fibroblast cell migration. Materials Science and Engineering C, 69. pp. 1256-1262. ISSN 1873-0191
|
Text
The effects of acoustic vibration on fibroblast cell migration.pdf - Accepted Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (1MB) | Preview |
Abstract
Cells are known to interact and respond to external mechanical cues and recent work has 9 shown that application of mechanical stimulation, delivered via acoustic vibration, can be 10 used to control complex cell behaviours. Fibroblast cells are known to respond to physical 11 cues generated in the extracellular matrix and it is thought that such cues are important 12 regulators of the wound healing process. Many conditions are associated with poor wound 13 healing, so there is need for treatments/interventions, which can help accelerate the wound 14 healing process. The primary aim of this research was to investigate the effects of mechanical 15 stimulation upon the migratory and morphological properties of two different fibroblast cells 16 namely; human lung fibroblast cells (LL24) and subcutaneous areolar/adipose mouse 17 fibroblast cells (L929). Using a speaker-based system, the effects of mechanical stimulation 18 (0-1600Hz for 5 minutes) on the mean cell migration distance (μm) and actin organisation 19 was investigated. The results show that 100Hz acoustic vibration enhanced cell migration for 20 both cell lines whereas acoustic vibration above 100Hz acoustic vibration was found to 21 decrease cell migration in a frequency dependent manner. Mechanical stimulation was also 22 found to promote changes to the morphology of both cell lines, particularly the formation of 23 lamellipodia and filopodia. Overall lamellipodia was the most prominent actin structure 24 displayed by the lung cell (LL24), whereas filopodia was the most prominent actin feature 25 displayed by the fibroblast derived from subcutaneous areolar/adipose tissue. Mechanical 26 stimulation at all the frequencies used here was found not to affect cell viability. These results 27 suggest that low-frequency acoustic vibration may be used as a tool to manipulate the 28 mechano-sensitivity of cells to manipulate cell migration and which may be used to aid 29 wound repair.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > RS Pharmacy and materia medica T Technology > TA Engineering (General). Civil engineering (General) |
| Divisions: | Pharmacy & Biomolecular Sciences Engineering Maritime & Mechanical Engineering (merged with Engineering 10 Aug 20) |
| Publisher: | Elsevier |
| Date Deposited: | 16 Sep 2016 10:58 |
| Last Modified: | 04 Sep 2021 12:35 |
| DOI or ID number: | 10.1016/j.msec.2016.07.037 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/4037 |
 |
View Item |