Lencione, D, Gehlen, MH, Trujillo, LN, Leitao, RCF and Albuquerque, RQ (2016) The spatial distribution of the photostability of thionine in zeolite L nanochannels investigated by Photobleaching Lifetime Imaging Microscopy. Photochemical and Photobiological Sciences, 15 (3). pp. 398-404. ISSN 1474-905X
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Abstract
Dye photobleaching is a photochemical reaction that can be investigated locally using fluorescence microscopy techniques. In this study, a user-friendly computational tool to assist photobleaching experiments called Photobleaching Lifetime Imaging Microscopy (PbLIM) is presented. With this tool it is possible to recover the photobleaching kinetics spatially, where a photobleaching lifetime is generated for each pixel of the image. Our model was applied to the photobleaching process of thionine encapsulated into the one-dimensional nano-channels of Zeolite L (ZL), from where we gained insight into the molecular oxygen distribution inside the ZL channels, as well as the detailed photobleaching of the confined thionine. © The Royal Society of Chemistry and Owner Societies 2016.
Item Type: | Article |
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Uncontrolled Keywords: | 0306 Physical Chemistry (Incl. Structural), 0601 Biochemistry And Cell Biology, 0299 Other Physical Sciences |
Subjects: | Q Science > QD Chemistry |
Divisions: | Pharmacy & Biomolecular Sciences |
Publisher: | Royal Society of Chemistry |
Date Deposited: | 29 Sep 2016 11:41 |
Last Modified: | 04 Sep 2021 04:11 |
DOI or ID number: | 10.1039/c5pp00418g |
URI: | https://researchonline.ljmu.ac.uk/id/eprint/4163 |
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