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Lanigan, PMP, Munro, I, Grace, EJ, Casey, DR, Phillips, J, Klug, DR, Ces, O and Neil, MAA (2012) Dynamical hologram generation for high speed optical trapping of smart droplet microtools. Biomedical Optics Express, 3. pp. 1609-1619. ISSN 2156-7085
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Abstract
This paper demonstrates spatially selective sampling of the plasma membrane by the implementation of time-multiplexed holographic optical tweezers for Smart Droplet Microtools (SDMs). High speed (>1000fps) dynamical hologram generation was computed on the graphics processing unit of a standard display card and controlled by a user friendly LabView interface. Time multiplexed binary holograms were displayed in real time and mirrored to a ferroelectric Spatial Light Modulator. SDMs were manufactured with both liquid cores (as previously described) and solid cores, which confer significant advantages in terms of stability, polydispersity and ease of use. These were coated with a number of detergents, the most successful based upon lipids doped with transfection reagents. In order to validate these, trapped SDMs were maneuvered up to the plasma membrane of giant vesicles containing Nile Red and human biliary epithelial (BE) colon cancer cells with green fluorescent labeled protein (GFP)-labeled CAAX (a motif belonging to the Ras protein). Bright field and fluorescence images showed that successful trapping and manipulation of multiple SDMs in x, y, z was achieved with success rates of 30-50% and that subsequent membrane-SDM interactions led to the uptake of Nile Red or GFP-CAAX into the SDM.
| Item Type: | Article |
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| Additional Information: | This paper was published in Biomedical Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://dx.doi.org/10.1364/BOE.3.001609. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law. |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Civil Engineering & Built Environment |
| Publisher: | OSA |
| Date Deposited: | 04 Mar 2015 15:49 |
| Last Modified: | 04 Sep 2021 14:39 |
| DOI or ID number: | 10.1364/BOE.3.001609 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/538 |
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