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Elosta, A, Slevin, M, Rahman, K and Ahmed, N (2017) Aged garlic has more potent antiglycation and antioxidant properties compared to fresh garlic extract in vitro. Scientific Reports, 7. ISSN 2045-2322
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Abstract
Protein glycation involves formation of early (Amadori) and late advanced glycation endproducts (AGEs) together with free radicals via autoxidation of glucose and Amadori products. Glycation and increased free radical activity underlie the pathogenesis of diabetic complications. This study investigated whether aged garlic has more potent antiglycation and antioxidant properties compared to fresh garlic extract in vitro in a cell-free system. Proteins were glycated by incubation with sugars (glucose, methylglyoxal or ribose) ±5–15 mg/mL of aged and fresh garlic extracts. Advanced glycation endproducts were measured using SDS-PAGE gels and by ELISA whereas Amadori products were assessed by the fructosamine method. Colorimetric methods were used to assess antioxidant activity, free radical scavenging capacity, protein-bound carbonyl groups, thiol groups and metal chelation activities in addition to phenolic, total flavonoid and flavonol content of aged and fresh garlic extracts. Aged garlic inhibited AGEs by 56.4% compared to 33.5% for an equivalent concentration of fresh garlic extract. Similarly, aged garlic had a higher total phenolic content (129 ± 1.8 mg/g) compared to fresh garlic extract (56 ± 1.2 mg/g). Aged garlic has more potent antiglycation and antioxidant properties compared to fresh garlic extract and is more suitable for use in future in vivo studies.
| Item Type: | Article |
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| Uncontrolled Keywords: | Science & Technology; Multidisciplinary Sciences; Science & Technology - Other Topics; ADVANCED GLYCATION ENDPRODUCTS; ALLIUM-SATIVUM L.; AQUEOUS EXTRACT; PROTEIN; PRODUCTS; DAMAGE; FRUIT; VIVO; TEA |
| Subjects: | Q Science > QD Chemistry R Medicine > RM Therapeutics. Pharmacology R Medicine > RV Botanic, Thomsonian, and eclectic medicine |
| Divisions: | Pharmacy & Biomolecular Sciences |
| Publisher: | Nature Publishing Group |
| Related URLs: | |
| Date Deposited: | 07 Apr 2017 08:47 |
| Last Modified: | 04 Sep 2021 11:44 |
| DOI or ID number: | 10.1038/srep39613 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/6222 |
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