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Bnyan, R, Khan, I, Ehtezazi, T, Saleem, IY, Gordon, S, O'Neill, F and Roberts, M (2019) Formulation and optimisation of novel transfersomes for sustained release of local anaesthetic. Journal of Pharmacy and Pharmacology. ISSN 0022-3573
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Abstract
Objective: To investigate the effect of formulation parameters on the preparation of transfersomes as sustained‐release delivery systems for lidocaine and to develop and validate a new high‐performance liquid chromatography (HPLC) method for analysis.
Method: Taguchi design of experiment (DOE) was used to optimise lidocaine‐loaded transfersomes in terms of phospholipid, edge activator (EA) and phospholipid : EA ratio. Transfersomes were characterised for size, polydispersity index (PDI), charge and entrapment efficiency (%EE). A HPLC method for lidocaine quantification was optimised and validated using a mobile phase of 30%v/v PBS (0.01 m) : 70%v/v Acetonitrile at a flow rate of 1 ml/min, detected at 255 nm with retention time of 2.84 min. The release of lidocaine from selected samples was assessed in vitro.
Key findings: Transfersomes were 200 nm in size, with PDI ~ 0.3. HPLC method was valid for linearity (0.1–2 mg/ml, R2 0.9999), accuracy, intermediate precision and repeatability according to ICH guidelines. The %EE was between 44% and 56% and dependent on the formulation parameters. Taguchi DOE showed the effect of factors was in the rank order : lipid : EA ratio ˃ EA type ˃ lipid type. Optimised transfersomes sustained the release of lidocaine over 24 h.
Conclusion: Sustained‐release, lidocaine‐loaded transfersomes were successfully formulated and optimised using a DOE approach, and a new HPLC method for lidocaine analysis was developed and validated.
| Item Type: | Article |
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| Additional Information: | This is the peer reviewed version of the following article: Bnyan, R. , Khan, I. , Ehtezazi, T. , Saleem, I. , Gordon, S. , O’Neill, F. and Roberts, M. (2019), Formulation and optimisation of novel transfersomes for sustained release of local anaesthetic. J Pharm Pharmacol. doi:10.1111/jphp.13149, which has been published in final form at http://dx.doi.org/10.1111/jphp.13149. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
| Uncontrolled Keywords: | 1115 Pharmacology and Pharmaceutical Sciences |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Pharmacy & Biomolecular Sciences |
| Publisher: | Wiley |
| Related URLs: | |
| Date Deposited: | 02 Sep 2019 08:26 |
| Last Modified: | 04 Sep 2021 08:58 |
| DOI or ID number: | 10.1111/jphp.13149 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/11241 |
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