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Mugenzi, LMJ, Menze, BD, Tchouakui, M, Wondji, MJ, Irving, H, Tchoupo, M, Hearn, J, Weedall, GD, Riveron, JM and Wondji, CS (2019) Cis-regulatory CYP6P9b P450 variants associated with loss of insecticide-treated bed net efficacy against Anopheles funestus. Nature Communications, 10. ISSN 2041-1723
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Abstract
Elucidating the genetic basis of metabolic resistance to insecticides in malaria vectors is crucial to prolonging the effectiveness of insecticide-based control tools including long lasting insecticidal nets (LLINs). Here, we show that cis-regulatory variants of the cytochrome P450 gene, CYP6P9b, are associated with pyrethroid resistance in the African malaria vector Anopheles funestus. A DNA-based assay is designed to track this resistance that occurs near fixation in southern Africa but not in West/Central Africa. Applying this assay we demonstrate, using semi-field experimental huts, that CYP6P9b-mediated resistance associates with reduced effectiveness of LLINs. Furthermore, we establish that CYP6P9b combines with another P450, CYP6P9a, to additively exacerbate the reduced efficacy of insecticide-treated nets. Double homozygote resistant mosquitoes (RR/RR) significantly survive exposure to insecticide-treated nets and successfully blood feed more than other genotypes. This study provides tools to track and assess the impact of multi-gene driven metabolic resistance to pyrethroids, helping improve resistance management.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Science & Technology; Multidisciplinary Sciences; Science & Technology - Other Topics; PYRETHROID RESISTANCE; GAMBIAE; GENE; OVEREXPRESSION; IDENTIFICATION; MECHANISMS; TOOL |
| Subjects: | Q Science > QH Natural history > QH426 Genetics R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Biological and Environmental Sciences (from Sep 19) |
| Publisher: | Nature Publishing Group |
| Related URLs: | |
| Date of acceptance: | 21 September 2019 |
| Date of first compliant Open Access: | 14 November 2019 |
| Date Deposited: | 14 Nov 2019 09:11 |
| Last Modified: | 04 Sep 2021 08:27 |
| DOI or ID number: | 10.1038/s41467-019-12686-5 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/11765 |
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