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Srisawat, K, Hesketh, KL, Cocks, MS, Strauss, JA, Edwards, BJ, Lisboa, PJ, Shepherd, SO and Burniston, JG (2019) Reliability of Protein Abundance and Synthesis Measurements in Human Skeletal Muscle. Proteomics, 20 (7). ISSN 1615-9853
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Abstract
We investigated the repeatability of dynamic proteome profiling (DPP), which is a novel technique for measuring the relative abundance (ABD) and fractional synthesis rate (FSR) of proteins in humans. LC-MS analysis was performed on muscle samples taken from male participants (n = 4) that consumed 4 × 50 ml doses of deuterium oxide (2 H2 O) per day for 14 d. ABD was measured by label-free quantitation and FSR was calculated from time-dependent changes in peptide mass isotopomer abundances. One-hundred and one proteins had at least 1 unique peptide and were used in the assessment of protein ABD. Fifty-four of these proteins met more stringent criteria and were used in the assessment of FSR data. The median (M), lower- (Q1 ) and upper-quartile (Q3 ) values for protein FSR (%/d) were M = 1.63, Q1 = 1.07, Q3 = 3.24. The technical CV of ABD data had a median value of 3.6% (Q1 1.7% - Q3 6.7%), whereas the median CV of FSR data was 10.1% (Q1 3.5% - Q3 16.5%). These values compare favorably against other assessments of technical repeatability of proteomics data, which often set a CV of 20% as the upper bound of acceptability. This article is protected by copyright. All rights reserved.
| Item Type: | Article |
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| Additional Information: | This is the peer reviewed version of the following article: Reliability of Protein Abundance and Synthesis Measurements in Human Skeletal Muscle, which has been published in final form at 10.1002/pmic.201900194. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
| Uncontrolled Keywords: | 06 Biological Sciences, 08 Information and Computing Sciences, 11 Medical and Health Sciences |
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QM Human anatomy Q Science > QP Physiology R Medicine > RC Internal medicine > RC1200 Sports Medicine |
| Divisions: | Sport & Exercise Sciences |
| Publisher: | Wiley |
| Related URLs: | |
| Date Deposited: | 04 Dec 2019 10:04 |
| Last Modified: | 04 Sep 2021 08:21 |
| DOI or ID number: | 10.1002/pmic.201900194 |
| URI: | https://researchonline.ljmu.ac.uk/id/eprint/11839 |
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