Ceresa, C, Hutton, S, Lajarin-Cuesta, M, Heaton, R, Hargreaves, IP, Fracchia, L and Diaz De Rienzo, MA (2020) Production of Mannosylerythritol Lipids (MELs) to be Used as Antimicrobial Agents against S. aureus ATCC 6538. Current Microbiology, 77. pp. 1373-1380. ISSN 0343-8651

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Abstract

Antimicrobial resistance (AMR) is a current major health issue, both for the high rates of resistance observed in bacteria that cause common infections and for the complexity of the consequences of AMR. Pathogens like Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Mycobacterium tuberculosis among others are clear examples of antibiotic-resistant threats. Biosurfactants have recently emerged as a potential new generation of anti-adhesive and anti-biofilm agents, mannosylerythritol lipids (MELs) are biosurfactants produced by a range of fungi. A range of structural variants of MELs can be formed and the proportion of each isomer in the fermentation depends on the yeast used, the carbon substrate used for growth and the duration of the fermentation. In order to allow assessment of the possible functions of MELs as antimicrobial molecules, small quantities of MEL were produced by controlled fermentation. Fermentations of the yeast Pseudozyma aphidis using rapeseed oil as a carbon source yielded up to 165gMELs/KgSubstrate. The MELs formed by this strain was a mixture of MEL-A, MEL-B, MEL-C and MEL-D. The MELs produced were tested against S. aureus ATCC 6538 on pre-formed biofilm and on co-incubation biofilm experiments on silicone discs; showing a disruption of biomass, reduction of the biofilm metabolic activity and a bacteriostatic/bactericidal effect confirmed by a release of oxygen uptake pO2, the reduction of Citrate Synthase activity and SEM. The results show that MELs are promising antimicrobial molecules for biomedical technological applications that could be studied in detail in large-scale systems and in conjunction with animal tissue models.

Item Type:	Article
Uncontrolled Keywords:	0605 Microbiology, 1108 Medical Microbiology
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisions:	Pharmacy & Biomolecular Sciences
Publisher:	Springer Verlag
Date Deposited:	25 Feb 2020 10:54
Last Modified:	13 Jan 2022 10:45
DOI or ID number:	10.1007/s00284-020-01927-2
URI:	https://researchonline.ljmu.ac.uk/id/eprint/12311

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