Adaptation of rat fast-twitch muscle to endurance activity is underpinned by changes to protein degradation as well as protein synthesis.

Hesketh, SJ; Sutherland, H; Lisboa, PJ; Jarvis, JC; Burniston, JG

Adaptation of rat fast-twitch muscle to endurance activity is underpinned by changes to protein degradation as well as protein synthesis.

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Hesketh, SJ, Sutherland, H, Lisboa, PJ, Jarvis, JC and Burniston, JG (2020) Adaptation of rat fast-twitch muscle to endurance activity is underpinned by changes to protein degradation as well as protein synthesis. FASEB Journal. ISSN 1530-6860

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Abstract

Muscle adaptations to exercise are underpinned by alterations to the abundance of individual proteins, which may occur through a change either to the synthesis or degradation of each protein. We used deuterium oxide (2 H2 O) labeling and chronic low-frequency stimulation (CLFS) in vivo to investigate the synthesis, abundance, and degradation of individual proteins during exercise-induced muscle adaptation. Independent groups of rats received CLFS (10 Hz, 24 h/d) and 2 H2 O for 0, 10, 20, or 30 days. The extensor digitorum longus (EDL) was isolated from stimulated (Stim) and contralateral non-stimulated (Ctrl) legs. Proteomic analysis encompassed 38 myofibrillar and 46 soluble proteins and the rates of change in abundance, synthesis, and degradation were reported in absolute (ng/d) units. Overall, synthesis and degradation made equal contributions to the adaptation of the proteome, including instances where a decrease in protein-specific degradation primarily accounted for the increase in abundance of the protein.

Item Type:	Article
Uncontrolled Keywords:	0601 Biochemistry and Cell Biology, 0606 Physiology, 1116 Medical Physiology
Subjects:	R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions:	Applied Mathematics (merged with Comp Sci 10 Aug 20) Sport and Exercise Sciences
Publisher:	Federation of American Society of Experimental Biology
Related URLs:	https://www.ncbi.nlm.nih.gov/pubmed/32598083
Date of acceptance:	21 May 2020
Date of first compliant Open Access:	9 July 2020
Date Deposited:	09 Jul 2020 09:58
Last Modified:	04 Sep 2021 07:02
DOI or ID number:	10.1096/fj.202000668RR
URI:	https://researchonline.ljmu.ac.uk/id/eprint/13275

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