Naaum, AM, Cusa, M, Singh, M, Bleicher, Z, Elliott, C, Goodhead, IB, Hanner, RH, Helyar, SJ, Mariani, S, Rice, JE, Wangh, LJ and Sanchez, JA (2021) Validation of FASTFISH-ID: A new commercial platform for rapid fish species authentication via universal closed-tube barcoding. Food Research International, 141. ISSN 0963-9969

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Abstract

Seafood represents up to 20% of animal protein consumption in global food consumption and is a critical dietary and income resource for the world's population. Currently, over 30% of marine fish stocks are harvested at unsustainable levels, and the industry faces challenges related to Illegal, Unregulated and Unreported (IUU) fishing. Accurate species identification is one critical component of successful stock management and helps combat fraud. Existing DNA-based technologies permit identification of seafood even when morphological features are removed, but are either too time-consuming, too expensive, or too specific for widespread use throughout the seafood supply chain. FASTFISH-ID is an innovative commercial platform for fish species authentication, employing closed-tube barcoding in a portable device. This method begins with asymmetric PCR amplification of the full length DNA barcode sequence and subsequently interrogates the resulting single-stranded DNA with a universal set of Positive/Negative probes labeled in two fluorescent colors. Each closed-tube reaction generates two species-specific fluorescent signatures that are then compared to a cloud-based library of previously validated fluorescent signatures. This novel approach results in rapid, automated species authentication without the need for complex, time consuming, identification by DNA sequencing, or repeated analysis with a panel of species-specific tests. Performance of the FASTFISH-ID platform was assessed in a blinded study carried out in three laboratories located in the UK and North America. The method exhibited a 98% success rate among the participating laboratories when compared to species identification via conventional DNA barcoding by sequencing. Thus, FASTFISH-ID is a promising new platform for combating seafood fraud across the global seafood supply chain. © 2021 The Authors

Item Type:	Article
Uncontrolled Keywords:	0904 Chemical Engineering, 0908 Food Sciences, 1111 Nutrition and Dietetics
Subjects:	G Geography. Anthropology. Recreation > GE Environmental Sciences S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions:	Biological & Environmental Sciences (from Sep 19)
Publisher:	Elsevier
Date Deposited:	24 Feb 2021 09:11
Last Modified:	04 Sep 2021 05:53
DOI or ID number:	10.1016/j.foodres.2020.110035
URI:	https://researchonline.ljmu.ac.uk/id/eprint/14506

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